摘要
目的 探讨血清微小核糖核酸-223(miR-223)、白细胞介素-6(IL-6)与复杂型热性惊厥(CFS)患儿脑损伤的关系。方法 选取124例CFS患儿(CFS组),根据是否发生脑损伤将CFS患儿分为脑损伤组39例和无脑损伤组85例,另选取46名同期健康儿童(对照组)为对照。采用实时荧光定量聚合酶链式反应与酶联免疫吸附法检测血清miR-223与IL-6水平。Spearman相关性法分析血清miR-223、IL-6在CFS患者血清中的相关性,多因素Logistic回归分析影响CFS患儿脑损伤的因素,受试者工作特征曲线分析血清miR-223、IL-6水平对CFS患儿脑损伤的预测价值。结果 与对照组比较,CFS组血清miR-223水平降低,IL-6水平升高(P均<0.05)。CFS患儿血清miR-223与IL-6水平呈负相关(r=-0.800,P<0.05)。124例CFS患儿脑损伤发生率为31.45%(39/124)。全身抽搐、惊厥发作次数≥2次、惊厥发作持续时间≥5 min、IL-6升高为CFS患儿脑损伤的独立危险因素,miR-223升高为独立保护因素(P均<0.05)。血清miR-223、IL-6水平联合预测CFS患儿脑损伤的曲线下面积为0.899,大于血清miR-223、IL-6水平单独预测的0.803、0.780(P均<0.05)。结论 血清miR-223水平降低和IL-6水平升高与CFS患儿脑损伤密切相关,血清miR-223、IL-6水平联合预测CFS患儿脑损伤的价值较高。
Objective To investigate the relationships between serum microRNA-223(miR-223),interleukin-6(IL-6) and brain damage in children with complex febrile seizures(CFS).Methods Totally 124 children with CFS(CFS group) were divided into the brain injury group of 39 cases and the non-brain injury group of 85 cases according to whether they had brain injury or not;meanwhile,46 healthy children at the same period(control group) were selected.Real-time fluorescence quantitative polymerase chain reaction and enzyme-linked immunosorbent assay were used to detect serum miR-223 and IL-6 expression.Spearman's correlation was used to analyze the correlation of serum miR-223 and IL-6 levels in the serum of CFS patients,multifactorial Logistic regression was used to analyze the factors affecting brain injury in children with CFS,and the predictive value of serum miR-223 and IL-6 levels in children with CFS was analyzed by using receiver operating characteristic(ROC) curve.Results Compared with the control group,serum miR-223 level decreased and IL-6 level increased in the CFS group(both P<0.05).Serum miR-223 was negatively correlated with IL-6level in children with CFS(r=-0.800,P<0.05).The incidence of brain injury in 124 children with CFS was 31.45%(39/124).Generalized convulsions,the number of convulsive seizures ≥2,duration of convulsive seizures ≥5 min,and elevated IL-6 were independent risk factors for brain injury in children with CFS,and elevated miR-223 was an independent protective factor(all P<0.05).The area under the curve of serum miR-223 combined with IL-6 level in prediction of brain injury of children with CFS was 0.899,which was greater than that(0.803,0.780) of serum miR-223 and IL-6 levels alone(both P<0.05).Conclusions Decreased serum miR-223 level and increased IL-6 level are closely associated with brain injury in children with CFS,and the combination of serum miR-223 and IL-6 levels is of high value in predicting brain injury in children with CFS.
作者
李婉晨
孙欣荣
LI Wanchen;SUN Xinrong(Graduate School,Xi'an Medical University,Xi'an 710068,China;不详)
出处
《山东医药》
CAS
2024年第10期21-25,共5页
Shandong Medical Journal
基金
陕西省卫生健康科研项目(2019D0063)。
作者简介
第一作者:李婉晨(1994-),女,主治医师,主要研究方向为呼吸、感染疾病的诊断及治疗。E-mail:slhospital_hx@163.com;通信作者:孙欣荣(1974-),女,博士研究生,主任医师,主要研究方向为呼吸、感染疾病的诊断及治疗。E-mail:13720533916@163.com。
