摘要
本研究旨在探讨短期饲喂高脂饲料对珍珠龙胆石斑鱼(Epinephelus fuscoguttatus ♀×Epinephelus lanceolatus♂)生长性能和抗氧化能力所产生的影响,以便于预估高脂饲料对石斑鱼生长可能产生的负面影响。在粗蛋白水平为48%的基础上分别配制脂肪水平为8%(EE8)、10%(EE10)、12%(EE12)、14%(EE14)、16%(EE16)、18%(EE18)和20%(EE20)的7组等氮饲料,饲喂初均重为(13.70±0.01)g的珍珠龙胆石斑鱼4周。实验结果表明,随着饲料粗脂肪水平的提升,增重率(WGR)和存活率(SR)无显著性变化(P>0.05),EE14~EE20组饲料系数(FCR)显著降低(P<0.05),而脏体比(VSI)显著增大(P<0.05),饲料粗脂肪超过16%后蛋白质效率(PER)显著上升(P<0.05)。EE14~EE20组腹肌粗脂肪(AMEEC)显著升高(P<0.05),EE16~EE20组的肝体比(HSI)显著增大(P<0.05)。与EE12组相比,EE8、EE10、EE18和EE20组的总胆固醇(T-CHO)显著升高(P<0.05),EE16~EE20组谷胱甘肽过氧化物酶(GSH-PX)活性显著下降(P<0.05),EE20组丙二醛(MDA)含量显著上升(P<0.05),EE8、EE16~EE20组的肝脏细胞空泡化面积和脂滴量显著增加(P<0.05)。脂肪合成基因g6pd、6gpd和me的相对表达量在EE20组显著上升(P<0.05),脂肪分解基因cpt-1、pparα相对表达量在EE16~EE20组显著上升(P<0.05)。研究结果表明,饲料粗脂肪超过16.77%时,会造成珍珠龙胆鱼肝脏脂肪沉积,降低肝脏抗氧化能力,使肝脏健康受损。
The aim of this experiment was to investigate the effects of high-fat diets on the short-term growth performance and antioxidant capacity of pearl grouper to help formulators and farmers anticipate the possible negative effects of high-fat diets on grouper growth and take effective measures in the formulation and feeding schedule of high-fat diets.Seven groups of isonitrogenous diets with fat levels of 8%(EE8),10%(EE10),12%(EE12),14%(EE14),16%(EE16),18%(EE18),and 20%(EE20)were fed to pearl grouper(initial mean weight:13.70±0.01g)for 4 weeks with a crude protein level of 48%.The experimental results showed no significant change in weight gain rate(WGR)and survival rate(SR)as the level of dietary ether extract increased(P>0.05),while the feed intake was significantly lower in the EE14-EE20 group(P<0.05);further,the viscerosomatic index(VSI)and protein efficiency ratio(PER)increased significantly(P<0.05)after the dietary ether extract exceeded 16%.The EE14-EE20 group had a significantly higher abdominal muscle ether extract(AMEEC)(P<0.05),and the EE16-EE20 group had a significantly greater hepatosomatic index(HSI)(P<0.05).Compared to the EE12 group,total cholesterol(T-CHO)was significantly higher in the EE8,EE10,EE18,and EE20 groups(P<0.05);glutathione peroxidase(GSH-PX)activity was significantly lower in the EE16-EE20 group(P<0.05);malondialdehyde(MDA)content was significantly higher in the EE20 group(P<0.05);and vacuolated area and hepatocytes in the EE8 and EE16-EE20 groups’lipid droplet amount were significantly increased(P<0.05).The relative expression of the lipid synthesis genes g6pd,6gpd,and me was significantly increased in the EE20 group(P<0.05),and the lipolytic genes cpt-1 and ppar α were significantly increased in the EE16-EE20 group(P<0.05).The results have shown that dietary ether extract above 16.77% causes fatty deposits in the liver of pearl amberjack,reducing the liver’s antioxidant capacity and impairing liver health.
作者
廖春燕
高文浩
李嘉辉
迟淑艳
LIAO Chunyan;GAO Wenhao;LI Jiahui;CHI Shuyan(School of Aquatic Sciences,Guangdong Ocean University,Zhanjiang 524088,China;Laboratory of Aquatic Animal Nutrition and Feed,Guangdong Ocean University,Zhanjiang 524088,China)
出处
《中国水产科学》
CAS
CSCD
北大核心
2023年第9期1080-1092,共13页
Journal of Fishery Sciences of China
基金
广东省基础与应用基础研究基金项目(2021A1515010634)。
关键词
珍珠龙胆石斑鱼
高脂饲料
生长性能
抗氧化能力
脂质代谢
Epinephelus fuscoguttatus ♀×E.lanceolatus ♂
high-fat diet
growth performance
antioxidant capacity
Lipid metabolism
作者简介
廖春燕(1996‒),女,硕士研究生,研究方向为水产动物营养与饲料.E-mail:2223586398@qq.com;通信作者:迟淑艳,教授,研究方向为水产动物营养与饲料.E-mail:chishuyan77@163.com。
