摘要
【目的】为明确香葱伴生对番茄青枯病及根际微生态特征的影响,揭示香葱伴生番茄降低青枯病发病率的机理。【方法】通过田间试验设置番茄单作和香葱伴生番茄2个处理,分别采集单作发病番茄(QK)、单作健康番茄(NBS)和香葱伴生番茄(BS)的根际土壤,应用16S rDNA高通量测序和液相色谱-质谱联用(LC-MS)技术,探究香葱伴生对番茄根际微生态特征的影响。【结果】田间试验结果表明,番茄单作处理和香葱伴生番茄处理的青枯病平均病株率分别为50.48、31.43%,香葱伴生番茄处理对番茄青枯病的防治效果为37.74%。16S rDNA高通量测序结果表明,BS、NBS根际土壤细菌群落相对丰度和多样性均显著高于QK;与QK相比,BS和NBS根际土壤细菌茄科雷尔氏菌属相对丰度显著降低,而芽孢杆菌属相对丰度升高;与NBS相比,BS根际土壤细菌茄科雷尔氏菌属相对丰度降低,芽孢杆菌属相对丰度显著提高。利用LC-MS技术共鉴定出番茄根际土壤代谢物586种,其中正离子模式代谢物320种,负离子模式代谢物266种,并将代谢物的功能注释至新陈代谢、遗传信息加工和环境信息加工等代谢通路;与NBS相比,BS根际土壤代谢物中的缬氨酸、L-天冬酰胺和柠檬酸的含量均显著降低,L-赖氨酸、谷氨酸、D-谷氨酰胺和异亮氨酸的含量也低于NBS,但差异未达到显著水平。【结论】香葱伴生番茄显著降低番茄青枯病田间发病率,改变了番茄根际土壤中细菌群落结构和番茄根际土壤代谢物含量。
【Objective】The aim of this study was to elucidate the effect of associated chives on bacterial wilt and micro-ecological characteristics of tomato rhizosphere,and to reveal the mechanism of reducing incidence of tomato bacterial wilt.【Method】Two treatments of tomato monoculture and tomatoes with chives were set for field experiments,and rhizosphere soils of diseased tomatoes in tomato monoculture treatment(QK),healthy tomatoes in tomato monoculture treatment(NBS)and tomatoes with chives treatment(BS)were collected.And the effects of companion chives on the micro-ecological characteristics of tomato rhizosphere were investigated by using 16S rDNA high-throughput sequencing and liquid chromatography-mass spectrometry.【Result】The results of field experiments showed that the average disease incidence of tomato bacterial wilt were 50.48%in tomato monoculture treatment and 31.43%in companion chives treatment.The control effect of companion chives on tomato bacterial wilt was 37.74%.According to the resulyts of 16S rDNA high-throughput sequencing,the relative abundance and diversity of bacterial communities in the rhizosphere soils of BS and NBS were significantly higher than those of QK.Compared with QK,the relative abundance of Ralstonia in the rhizosphere soils of BS and NBS was significantly lower than that of QK,and the relative abundance of Bacillus was higher than that of QK.Moreover,compared with NBS,the relative abundance of Ralstonia in the rhizosphere soil of BS was reduced,and the relative abundance of Bacillus was significantly enhanced.A total of 586 metabolites in tomato rhizosphere soil were identified by using LC-MS,including 320 positive ion mode metabolites and 266 negative ion mode metabolites.The functions of metabolites were annotated to metabolic pathways such as metabolism,genetic information processing and environmental information processing.Compared with NBS,the contents of valine,L-asparagine and citric acid in BS rhizosphere soil were significantly lower than those in NBS,and the contents of L-lysine,glutamic acid,D-glutamine and isoleucine in rhizosphere soil of BS were also lower than those of NBS,though,the difference was not significant.【Conclusion】The companion chives significantly reduced the disease incidence of tomato bacterial wilt in the field,and changed the bacterial community structure and metabolite contents in tomato rhizosphere soil.
作者
佘小漫
姚挺
黄真珍
何自福
SHE Xiaoman;YAO Ting;HUANG Zhenzhen;HE Zifu(Institute of Plant Protection,Guangdong Academy of Agricultural Sciences/Key Laboratory of Green Prevention and Control on Fruits and Vegetables in South China,Ministry of Agriculture and Rural Affairs/Guangdong Key Laboratory of High Technology for Plant Protection,Guangzhou 510640,China;Guangdong Agricultural Technology Promotion Center,Guangzhou 510145,China)
出处
《广东农业科学》
CAS
2024年第1期10-20,共11页
Guangdong Agricultural Sciences
基金
广东省重点领域研发计划项目(2020B0202090002)
广东省现代农业产业共性关键技术研发创新团队建设(2023KJ113)
广东省农业科学院科技创新战略专项资金(高水平农科院建设)(R2023PY-JG011)
广东省农业科学院“十四五”学科团队建设项目(202105TD)
广东省乡村振兴战略专项(粤农农计〔2023〕13号)。
关键词
香葱伴生
番茄青枯病
根际土壤细菌
根际土壤代谢物
防效
companion chive
tomato bacterial wilt
rhizosphere soil bacteria
rhizosphere soil metabolite
control efficiency
作者简介
佘小漫(1981-),博士,硕士生导师,广东省农业科学院植物保护研究所研究员。主要从事植物病理学、蔬菜病害诊断和绿色防控等研究。主持和参与国家科技支撑计划、国家重点研发计划子课题、国家自然科学基金、现代农业产业技术体系、广东省自然科学基金等项目30余项,发表论文30余篇,其中SCI论文12篇。获各级科技成果奖励20项,授权国家发明专利8项,参与制定标准3项,参编专著3部。担任广东省植物病理学会副秘书长、广东省植物保护学会理事、广东省现代农业产业体系创新团队专题专家。E-mail:lizer126@126.com;通信作者:黄真珍(1982-),女,硕士,高级农艺师,研究方向为农业技术推广,E-mail:lzsftgk@163.com;通信作者:何自福(1966-),男,博士,研究员,研究方向为植物病理学,E-mail:hezf@gdppri.com。
