摘要
以分蘖洋葱和番茄为材料,采用高通量测序研究伴生分蘖洋葱对番茄根际土壤细菌和真菌群落的组成和结构的影响。结果表明:与番茄单作相比,分蘖洋葱伴生处理改变了番茄根际土壤细菌和真菌群落的组成,变形菌门(Proteobacteria)、拟杆菌门(Bacteroidetes)、放线菌门(Actinobacteria)、酸杆菌门(Acidobacteria)、厚壁菌门(Firmicutes)、浮霉菌门(Planctomycetes)和绿弯菌门(Chloroflexi)为优势细菌门,子囊菌门(Ascomycota)、接合菌门(Zygomycota)和担子菌门(Basidiomycota)为优势真菌门;在纲水平上,分蘖洋葱伴生处理显著提高了具有有益潜力的α-变形菌纲(Alphaproteobacteria)的相对丰度,显著或极显著降低了粪壳菌纲(Sordariomycetes)和散囊菌纲(Eurotiomycetes)的相对丰度;在目水平上,分蘖洋葱伴生处理显著提高了鞘脂单胞菌目(Sphingomonadales)和丙酸杆菌目(Propionibacteriales)的相对丰度,显著或极显著降低了小囊菌目(Microascales)、散囊菌目(Onygenales)和炭角菌目(Xylariales)的相对丰度;在科水平上,分蘖洋葱伴生处理显著或极显著提高了类诺卡氏菌科(Nocardioidaceae)、微杆菌科(Microbacteriaceae)、鞘脂单胞菌科(Sphingomonadaceae)、Blrii41和毛球壳科(Lasiosphaeriaceae)的相对丰度,显著或极显著降低了小囊菌科(Microascaceae)和爪甲团囊菌科(Onygenaceae)的相对丰度;在属水平上,分蘖洋葱伴生处理显著降低了具有致病潜力的假霉样真菌属(Pseudallescheria)、Kernia和Wardomyces的相对丰度;但是对根际土壤细菌和真菌群落结构和微生物多样性无显著影响。
Taking tillering onion and tomato as material,this paper studied the effects of concomitant tillering onion on tomato rhizosphere soil bacterial and fungal community constitution and structure using highthroughput sequencing.Results showed that comparing with tomato monoculture,concomitant treatment with tillering onion could change the bacterial and fungal community constitution in tomato rhizosphere soil.Proteobacteria,Bacteroidetes,Actinobacteria,Acidobacteria,Firmicutes,Planctomycetes,Chloroflexi were advantage bacteriophyta.Ascomycota,Zygomycota,and Basidiomycota were dominant fungal phylum.At class level,the treatment of concomitant tillerng onion could significantly enhance the relative abundance of Alphaproteobacteria with beneficial potential,while reduced that of Sordariomycete and Eurotiomycetes.At the order level,concomitant tillering onion treatment could significantly enhance the relative abundance of Sphingomonadales and Propionibacteriales,while reduce that of Microascales,Onygenales and Xylariales.At the family level,concomitant tillering onion treatment could significantly enhance the relative abundance of Nocardioidaceae,Microbacteriaceae,Sphingomonadaceae,Blrii41 and Lasiosphaeriaceae,while reduce that of Microascaceae and Onygenaceae.At the genus level,concomitant tillering onion treatment could significantly reduce the relative abundance of fake allescheria with pathopolesis potential—Pseudallescheria,Kernia and Wardomyces.However,there was no significant effect on rhizosphere soil bacterial,fungal community structure and biodiversity.
作者
付彦祥
李乃荟
刘佳遥
吴凤芝
FU Yan-xiang;LI Nai-hui;LIU Jia-yao;WU Feng-zhi(College of Horticulture and landscape Architecture,Northeast Agricultural University,Harbin 150030,Heilongjiang,China;Jilin Academy of Agricultural Sciences,Changchun 130033,Jilin,China)
出处
《中国蔬菜》
北大核心
2020年第6期49-57,共9页
China Vegetables
基金
国家自然科学基金项目(3167200,31872156)。
关键词
番茄
分蘖洋葱
单作
伴生
根际土壤
微生物群落结构
Tomato
Tillering onion
Monoculture
Concomitant
Rhizosphere soil
Microbial community structure
作者简介
付彦祥,男,硕士研究生,专业方向:设施园艺与蔬菜生理生态,E-mail:673367316@qq.com;通讯作者:吴凤芝,女,教授,博士生导师,专业方向:设施园艺与蔬菜生理生态,E-mail:fzwu2006@aliyun.com。
