摘要
目的采用超高效液相色谱法建立3种蔷薇科种子类药材的特征图谱,并结合化学计量学进行分析,为甜杏仁、苦杏仁、桃仁的鉴别及质量控制提供依据。方法选择Agilent ZORBAX SB-Aq(2.1 mm×100 mm,1.8μm)为色谱柱,以乙腈为流动相A,0.2%磷酸溶液为流动相B进行梯度洗脱,流速为0.3 mL·min^(-1),柱温为25℃,检测波长为210 nm,共采集3种蔷薇科药材35批,建立特征谱图并进行相似度评价,结合聚类分析、正交偏最小二乘法判别分析对苦杏仁、甜杏仁、桃仁的化学成分进行研究。结果3种蔷薇科种子类药材的特征图谱具有明显差异,可通过峰2、峰4区分苦杏仁、甜杏仁和桃仁;采用质谱指认结合相关文献研究初步指认了特征图谱中的5种成分,并通过对照品对比进一步指认其中2种成分为苦杏仁苷和野黑樱苷;通过聚类分析能准确区分3种药材,且其种内相似度较高,种间相似度较低;正交偏最小二乘判别分析结果与特征图谱分析、聚类分析一致,并筛选出苦杏仁苷为3种药材的差异性标志物。结论建立的分析方法操作简便,可快速、准确区分苦杏仁、甜杏仁、桃仁,为3种蔷薇科药材的鉴别及质量控制提供参考。
Objective To establish UPLC characteristic chromatogram of 3 Rosaceae medicinal materials to identify and control the quality of Armeniacae Semen Amarum,sweet apricot kernel and Persicae Semen combined with chemometrics analysis.Methods The gradient elution was performed on the Agilent ZORBAX SB-Aq column(2.1 mm×100 mm,1.8μm)with the mobile phase of acetonitrile-0.2%phosphoric acid solution at 0.3 mL·min^(-1).The column temperature was 25℃.Totally 35 batches of 3 Rosaceae medicinal materials were collected,the characteristic spectrum was established and the similarity was evaluated.The chemical components of Armeniacae Semen Amarum,sweet apricot kernel and Persicae Semen were studied by cluster analysis and orthogonal partial least squares discriminant analysis.Results The characteristic chromatograms of the 3 seed medicinal materials of Rosaceae were obviously different.The Armeniacae Semen Amarum,sweet apricot kernel and Persicae Semen could be distinguished by peak 2 and 4.Totally 5 characteristic peaks were preliminarily identified through MS identification combined with relevant literature research,and 2 components were further identified as amygdalin and prunasin by comparison with the reference substances.Cluster analysis accurately distinguished 3 types of medicinal materials,with high intra species similarity and low inter species similarity.The results of orthogonal partial least squares discriminant analysis were consistent with those of characteristic chromatogram analysis and cluster analysis,and amygdalin was selected as a Q-marker for the 3 medicinal materials.Conclusion The method is simple,rapid and accurate,which can provide reference for the identification and quality control of 3 Rosaceae medicinal materials.
作者
叶聪
谢翡翡
李国卫
胡绮萍
童培珍
吴润松
罗文汇
孙冬梅
YE Cong;XIE Fei-fei;LI Guo-wei;HU Qi-ping;TONG Pei-zhen;WU Run-song;LUO Wen-hui;SUN Dong-mei(Guangzhou University of Traditional Chinese Medicine,Guangzhou 510006;Guangdong Yifang Pharmaceutical Co.,Ltd.,Guangdong Key Laboratory of Traditional Chinese Medicine Formula Granules Enterprise,Foshan Guangdong 528244)
出处
《中南药学》
CAS
2024年第2期315-321,共7页
Central South Pharmacy
基金
国家工信部2022年产业技术基础公共服务平台项目-中药全产业链质量技术服务平台(No.2022-230-221)。
关键词
苦杏仁
桃仁
甜杏仁
特征图谱
化学计量学
质量评价
Armeniacae Semen Amarum
Persicae Semen
sweet apricot kerne
characteristic chromatogram
chemometrics
quality evaluation
作者简介
叶聪,男,在读硕士研究生,主要从事中药质量评价研究,email:630985790@qq.com;通信作者:孙冬梅,女,教授,主要从事中药新药研发及中药质量评价研究,email:1471237855@qq.com。
