摘要
目的探究白芨多糖对大鼠跨区皮瓣choke区的影响和机制。方法将32只SD大鼠随机分成两组:对照组,给予生理盐水;给药组,给予白芨多糖生理盐水溶液。手术结扎胸背动脉和肋间后动脉,保留髂腰动脉。术后7 d,统计皮瓣存活率,血管造影观察choke动脉生长情况,HE染色显微镜下观察choke区微血管密度和动脉内径,Western blot检测choke区皮瓣组织血管内皮生长因子(VEGF)蛋白表达水平。结果术后7 d,给药组皮瓣存活率高于对照组,(86.1±1.4)%vs(61.3±4.0)%,P<0.0001;给药组髂腰动脉直径更粗,(681.60±35.48)μm vs(446.98±21.61)μm,P<0.0001;血管向皮瓣远端延伸更长,(6.50±0.33)cm vs(5.41±0.38)cm,P<0.0001;给药组choke区微血管密度更高(11.8±1.5)个vs(6.7±1.3)个,P<0.0001;动脉内径更大,(60.14±6.99)μm vs(28.01±5.07)μm,P<0.0001;VEGF蛋白表达水平增加,P<0.0001。结论白芨多糖可促进皮瓣成活,机制与调节choke区血管新生、扩张和VEGF表达有关。
Objective To investigate the effect and mechanism of Bletilla striata polysaccharides on the choke-area of cross-boundary skin flap in rats.Methods Thirty-two SD rats were randomly divided into two groups:a control group,was given physiological saline;a treatment group,was given physiological saline solution of Bletilla striata polysaccharides.The thoracodorsal artery and posterior intercostal artery were ligated surgically,preserving the iliac lumbar artery.The survival rate of the flap was counted at 7 days after operation.The growth of choke artery was observed by angiography.The microvessel density and arterial intraluminal diameter of choke area were observed by HE staining microscope,and the expression level of vascular endothelial growth factor(VEGF)protein in the flap tissue of choke area was detected by Western blot.Results At day 7 after operation,the survival rate of the flap in the treatment group was higher than that in the control group[(86.1±1.4)%vs(61.3±4.0)%,P<0.0001].The diameter of the iliopsoas artery in the treatment group was thicker than that of the control group(681.60±35.48 vs 446.98±21.61μm,P<0.0001),and the blood vessels extended longer towards the distal end of the flap(6.50±0.33 vs 5.41±0.38 cm,P<0.0001).The microvessel density in choke area was higher in the treatment group[(11.8±1.5)vs(6.7±1.3),P<0.0001],with a larger arterial lumen diameter in choke area(60.14±6.99 vs 28.01±5.07μm,P<0.0001).The expression level of VEGF protein increased(P<0.0001).Conclusions Bletilla polysaccharides can promote the survival of flap,through the regulation of angiogenesis,dilation and VEGF expression in the Chokearea.
作者
乐钦
陈荣丽
熊婧熙
王婷怡
蔡志恒
易秋实
曾心怡
Yue Qin;Chen Rongli;Xiong Jingxi;Wang Tingyi;Cai Zhiheng;Yi Qiushi;Zeng Xinyi(Yangtze University Health Science Center,Jingzhou 434023,Hubei Province,China)
出处
《中国临床解剖学杂志》
CSCD
北大核心
2024年第1期59-64,共6页
Chinese Journal of Clinical Anatomy
基金
长江大学医学部2022年大学生创新训练项目
国家自然科学基金项目(82271514)。
关键词
白芨多糖
choke区
跨区皮瓣
血管新生
Bletilla striata polysaccharide
Choke area
Cross-boundary flap
Angiogenesis
作者简介
乐钦(2003-),女,湖北咸宁人,在读本科,主要从事显微外科应用解剖学研究,E-mail:2542003245@qq.com;通讯作者:曾心怡,硕士,中级实验员,E-mail:778578952@qq.com。
