摘要
文冠果种子为文冠果树的成熟种子,在食品和制药领域有广泛的应用。为了比较分析文冠果种子不同部位的化学成分,并挖掘非药用部位的潜在价值和研究前景,该研究采用超高效液相色谱串联四极杆静电场轨道阱高分辨质谱法(UHPLC-Q-Orbitrap HRMS)检测不同部位的化学成分,从文冠果种子中初步鉴定了82种成分,包括5种氨基酸类,4种多酚类,3种苯丙酸类,7种有机酸,15种黄酮类化合物,6种糖苷类和23种皂苷类等。将不同部位的质谱结果进行质谱分子网络(MN)分析,发现种仁、种皮和种壳的成分具有较大的差异,种仁中皂苷类和黄酮类成分在种类和含量上优于种皮和种壳。根据不同部位多批次样品的色谱峰进行多元统计分析,并结合高效液相色谱法测定分析了4种共有差异性成分,结果显示种仁、种皮和种壳中秦皮苷的平均质量分数分别为0.1836、0.8874、1.4401 mg·g^(-1),儿茶素的平均质量分数分别为0.0358、0.1241、0.0445 mg·g^(-1),秦皮素的平均质量分数分别为0.0329、0.0720、0.2215 mg·g^(-1),表儿茶素的平均质量分数分别为0.4359、2.1147、0.2597 mg·g^(-1),测定结果表明了儿茶素和秦皮素在各部位中含量均较低,秦皮苷在种皮和种壳中含量较高,表儿茶素在种仁和种皮中含量较高,因此,种皮和种壳具有一定的开发价值。该研究对文冠果种子不同部位的化学成分进行快速分析比较,为文冠果种子的药效物质基础研究和临床应用提供了实验依据。
Xanthoceras sorbifolium seeds have a wide range of applications in the food and pharmaceutical industries.To compare and analyze the chemical compositions of different parts of X.sorbifolium seeds and explore the potential value and research prospects of non-medicinal parts,this study used ultra-high-performance liquid chromatography quadrupole Orbitrap high-resolution mass spectrometry(UHPLC-Q-Orbitrap HRMS)to detect the chemical composition of various parts of the seeds.A total of 82 components were preliminary identified from X.sorbifolium seeds,including 5 amino acids,4 polyphenols,3 phenylpropionic acids,7 organic acids,15 flavonoids,6 glycosides,and 23 saponins.Mass spectrometry molecular networking(MN)analysis was conducted on the results from different parts of the seeds,revealing significant differences in the components of the seed kernel,seed coat,and seed shell.The saponins and flavonoids in the seed kernel were superior in terms of variety and content to those in the seed coat and shell.Based on the chromatographic peaks of different parts from multiple batches of samples,multivariate statistical analysis was carried out.Four differential components were determined using HPLC,and the average content of these components in the seed kernel,seed coat,and seed shell were as follows:0.1836,0.8874,and 1.4401 mg·g^(-1)for fraxin;0.0358,0.1241,and 0.0445 mg·g^(-1)for catechin;0.0329,0.0720,and 0.2215 mg·g^(-1)for fraxetin;0.4359,2.1147,and 0.2597 mg·g^(-1)for epicatechin.The results showed that catechin and fraxetin had relatively low content in all parts,while fraxin had higher content in the seed coat and seed shell,and epicatechin had higher content in the seed kernel and seed coat.Therefore,the seed coat and seed shell possess certain development value.This study provides rapid analysis and comparison of the chemical compositions of different parts of X.sorbifolium seeds,which offers an experimental basis for the research and clinical application of medicinal substances in X.sorbifolium seeds.
作者
院军
张宏伟
张振凌
吴亚宁
李雅静
YUAN Jun;ZHANG Hong-wei;ZHANG Zhen-ling;WU Ya-ning;LI Ya-jing(School of Pharmacy,Henan University of Chinese Medicine,Zhengzhou 450046,China;Henan Research Center for Special Processing Technology of Chinese Medicine,Zhengzhou 450046,China;Co-construction Collaborative Innovation Center for Chinese Medicine and Respiratory Diseases by Henan&Education Ministry,Zhengzhou 450046,China)
出处
《中国中药杂志》
CSCD
北大核心
2023年第23期6347-6360,共14页
China Journal of Chinese Materia Medica
基金
国家公益性行业专项“中药炮制技术传承基地建设”项目(00104296)
河南省药品监督管理局科技计划专项(2020DB222)
郑州市协同创新专项(2023XTCX041)。
作者简介
通信作者:张振凌,教授,博士生导师,研究方向为中药炮制机制及饮片标准化,E-mail:zhangzl6758@163.com;院军,硕士研究生,研究方向为中药炮制技术,E-mail:957019561@qq.com。
