摘要
为了研究外膜蛋白OmpU对副溶血弧菌耐药性的影响,作者构建了副溶血弧菌中高丰度外膜蛋白OmpU缺失的突变株ΔompU。通过最小抑菌浓度(MIC)检测发现ΔompU对12种抗生素的耐药性发生变化,其中对氨苄青霉素的耐药性增加幅度最高。定量反转录PCR显示,在氨苄青霉素刺激下,野生型副溶血弧菌与ΔompU中肽聚糖合成基因转录水平普遍下调,而编码β内酰胺酶的基因VP_RS17515在野生型中转录水平高于ΔompU。结果表明,副溶血弧菌通过表达β内酰胺酶并降低肽聚糖合成速度的方式应对氨苄青霉素刺激,而OmpU通过改变细胞膜通透性来影响氨苄青霉素的渗入。
For understanding the effect of OmpU on antibiotics resistance of Vibrio parahaemolyticus,a deletion mutant strain of the highly abundant outer membrane protein(OmpU)in Vibrio parahaemolyticus,designated asΔompU,was constructed in this study.Minimum inhibitory concentration(MIC)assay revealed altered antibiotic resistances ofΔompU to 12 antibiotics,with the highest increase observed in resistance to ampicillin.Quantitative reverse transcription PCR showed a general downregulation in the transcription level of peptidoglycan synthesis genes in both wild-type(WT)andΔompU cells under ampicillin stimulation.However,the gene VP_RS17515 encodingβ-lactamase exhibited the higher transcription level in WT strains thanΔompU.The results suggest that V.parahaemolyticus responds to ampicillin through expressingβ-lactamase and reducing peptidoglycan synthesis rates,while OmpU changes the cell membrane permeability to affect the penetration of ampicillin.
作者
孟祥宇
王建莉
黄丹阳
周晴
檀昕
王小元
MENG Xiangyu;WANG Jianli;HUANG Danyang;ZHOU Qing;TAN Xin;WANG Xiaoyuan(School of Biotechnology,Jiangnan University,Wuxi 214122,China;School of Food Science and Technology,Jiangnan University,Wuxi 214122,China;State Key Laboratory of Food Science and Resources,Jiangnan University,Wuxi 214122,China)
出处
《食品与生物技术学报》
CAS
CSCD
北大核心
2023年第12期62-71,共10页
Journal of Food Science and Biotechnology
基金
国家重点研发计划项目(2017YFC1600102)。
关键词
副溶血弧菌
外膜蛋白
氨苄青霉素
耐药机制
Vibrio parahaemolyticus
outer membrane protein
ampicillin
antibiotic resistance mechanism
作者简介
通信作者:王小元(1965—),男,博士,教授,博士研究生导师,主要从事微生物细胞膜和细胞壁关键分子组成、结构及功能研究。E-mail:xwang@jiangnan.edu.cn。
