摘要
【目的】探究白藜芦醇(resveratrol,Res)对黄曲霉毒素B_(1)(aflatoxin B_(1),AFB_(1))致兔肾脏损伤的保护作用。【方法】将21只40日龄的家兔随机分为3组,每组7只,分别为对照组、AFB_(1)组和AFB_(1)+Res组,其中对照组饲喂基础饲粮,AFB_(1)组饲喂含0.3 mg/kg BW AFB_(1)的基础饲粮、AFB_(1)+Res组饲喂含0.3 mg/kg BW AFB_(1)和30 mg/kg BW Res的基础饲粮,试验期21 d。试验结束后观察家兔肾脏组织病理变化,检测血清中肌酐(creatinine,CRE)、尿素氮(urea nitrogen,BUN)和尿酸(uric acid,UA)含量,检测肾脏组织中谷胱甘肽过氧化物酶(glutathione peroxidase,GSH-Px)、超氧化物歧化酶(superoxide dismutase,SOD)、总抗氧化能力(total antioxidant capacity,T-AOC)和丙二醛(malonaldehyde,MDA)含量/活性。【结果】与对照组相比,AFB_(1)组家兔肾脏可见肾小囊腔扩张、肾小管上皮细胞排列紊乱、细胞核模糊和脱落,且肾脏中糖原沉积增多;血清中CRE、BUN和UA含量均显著升高(P<0.05);肾脏组织中GSH-Px、SOD活性和T-AOC均显著降低(P<0.05),MDA含量显著升高(P<0.05)。与AFB_(1)组相比,AFB_(1)+Res组家兔肾脏组织结构损伤得到缓解,且糖原沉积减少;血清中CRE、BUN和UA含量均显著降低(P<0.05);肾脏组织中GSH-Px、SOD活性和T-AOC均显著升高(P<0.05),MDA含量显著降低(P<0.05)。【结论】饲料中添加Res可以通过提高兔肾脏抗氧化能力、调节氧化应激水平而有效缓解AFB_(1)引起的兔肾脏损伤。
【Objective】This study explored the protective effect of resveratrol(Res)on kidney injury in rabbits induced by aflatoxin B_(1)(AFB_(1)).【Method】21 rabbits aged 40 days were randomly divided into 3 groups,7 rabbits in each group,which were control group,AFB_(1) group and AFB_(1)+Res group.The rabbits in control group was fed with basic diet,in AFB_(1) group was fed with basal diet containing 0.3 mg/kg BW AFB_(1),in AFB_(1)+Res group was fed with basal diet containing 0.3 mg/kg BW AFB_(1) and 30 mg/kg BW Res.The experiment period was 21 days.At the end of the experiment,the pathological changes of kidney tissues in rabbit were observed,and the contents of creatinine(CRE),urea nitrogen(BUN)and uric acid(UA)in serum were detected.And the glutathione peroxidase(GSH-Px),superoxide dismutase(SOD),total antioxidant capacity(T-AOC)and malonaldehyde(MDA)contents/activeties in kidney tissues were evaluated.【Result】Compared with the control group,the pathological changes in the kidneys of rabbits in AFB_(1) group included narrowed capsular space,disordered arrangement of renal tubular epithelial cells,fuzzy and exfoliated nuclei,and glycogen accumulation in the kidneys.The contents of CRE,BUN and UA in serum of rabbits in AFB_(1) group were significantly increased(P<0.05).The activities of GSH-Px,SOD and T-AOC in the kidney tissues of rabbits in AFB_(1) group were significantly decreased(P<0.05),and the level of MDA was significantly increased(P<0.05).Compared with AFB_(1) group,for the rabbits in Res+AFB_(1) group,the renal tissue structure damage in kidney was alleviated and the accumulation of glycogen was significantly reduced,the contents of CRE,BUN and UA in serum were significantly decreased,the levels of GSH-Px,SOD and T-AOC in the kidney tissue were significantly increased(P<0.05),and the level of MDA was significantly decreased(P<0.05).【Conclusion】Adding Res in the diet could effectively alleviate the kidney damage caused by AFB_(1) via improving the antioxidant capacity to regulate the level of oxidative stress of kidney in the rabbit.
作者
刘潍萁
张伊阳
张自强
丁轲
刘玉梅
LIU Weiqi;ZHANG Yiyang;ZHANG Ziqiang;DING Ke;LIU Yumei(College of Animal Science and Technology,Henan University of Science and Technology,Luoyang 471000,China)
出处
《中国畜牧兽医》
CSCD
北大核心
2024年第1期153-159,共7页
China Animal Husbandry & Veterinary Medicine
基金
河南省重点研发与推广专项(202102110093)
校企合作项目(横20210018)。
作者简介
刘潍萁,E-mail:210321251341@stu.haust.edu.cn。;通信作者:刘玉梅,E-mail:yumeiliu@haust.edu.cn。
