摘要
为探究复方中草药四味穿心莲益生菌发酵产物(FPSCS)对鸡大肠杆菌的作用,采用微孔-平板法测定其对O_(2)型鸡大肠杆菌的最小抑菌浓度(MIC)和最小杀菌浓度(MBC),以O_(2)型鸡大肠杆菌半数致死量(LD_(50))感染SPF雏鸡制备病理模型,随机分为FPSCS高、中、低剂量组,硫酸新霉素组,四味穿心莲散组,益生菌组及对照组,统计总有效率、治愈率;ELISA试剂盒检测各组动物外周血液中炎症因子的含量。结果显示:FPSCS体外对O_(2)型鸡大肠杆菌的MIC和MBC均为31.25 mg/mL,口服FPSCS剂量为0.1~0.5 g/(次·只)的总有效率为73.33%~83.33%、治愈率为70%~80%,均显著高于四味穿心莲散组和益生菌组(P<0.05),且治疗初期可显著降低促炎因子TNF-α、IL-1β、IL-6的含量(P<0.05),显著升高IFN-γ的含量(P<0.05)。由此表明,FPSCS可通过抑杀O_(2)型鸡大肠杆菌和降低鸡外周血促炎因子的含量而发挥较好的抗菌活性。
In order to investigate the effect of probiotic fermentation products(FPSCS)of compound traditional Chinese medicine Siwei Chuanxinlian(FPSCS)on chicken Escherichia coli(E.coli),the minimum inhibitory concentration(MIC)and minimum bactericidal concentration(MBC)of FPSCS against type O_(2) E.coli were determined by microporous plate method,and the specific pathogen free(SPF)chicken were infected with half lethal dose(LD_(50))of type O_(2) E.coli to prepare pathological model.They were randomly divided into high-dose group,medium-dose group and low-dose group of FPSCS,neomycin sulfate group,Siwei Chuanxinlian San group,probiotics group and control group.The total effective rate and cure rate of each group were analyzed,and the contents of inflammatory factors in peripheral blood of tested animals were detected by enzyme linked immunosorbent assay(ELISA)kits.The results showed that the MIC and MBC of FPSCS against Escherichia coli type O_(2) were 31.25 mg/mL in vitro,and the total effective rate of 0.1~0.5 g/(time·only)of FPSCS was 73.33%~83.33%and the cure rate was 70%~80%,which were significantly higher than those of Siwei Chuanxinlian San group and probiotics group(P<0.05).At the beginning of treatment,the contents of TNF-α,IL-1βand IL-6 were significantly decreased(P<0.05),and the contents of IFN-γwere significantly increased(P<0.05).These conclusion indicated that FPSCS could exert good antibacterial activity by inhibiting type O_(2) E.coli and reducing the content of proinflammatory factor in chicken.
作者
单文琪
江庆国
张述阳
谭艳平
周铁忠
王宏军
SHAN Wenqi;JIANG Qingguo;ZHANG Shuyang;TAN Yanping;ZHOU Tiezhong;WANG Hongjun(College of Animal Science and Veterinary Medicine,Jinzhou Medical University,Jinzhou,Liaoning Province 121001,China;Dalian Sanyi Animal Medicine Co.,LTD.,Dalian,Liaoning Province 116036,China;Shenyang Shenhua Institute Test Technology Co.,LTD.,Shenyang,Liaoning Province 110141,China)
出处
《中国饲料》
北大核心
2024年第1期89-92,共4页
China Feed
基金
辽宁省民生科技计划项目(2021JH2/10200009)
辽宁省科学技术计划农业重大专项(2020JH1/10200003)
辽宁省普通本科高等学校校际合作重大科研项目(辽教发202028-59)。
关键词
四味穿心莲散
益生菌
中草药发酵
鸡
大肠杆菌
Siwei Chuanxinlian San
probiotics
fermentation product of traditional Chinese medicine
chicken
Escherichia coli
作者简介
单文琪(1999-),女,硕士生,兽医专业,主要从事中药发酵,E-mail:1186140464@qq.com;通讯作者:王宏军(1977-),男,博士,教授,主要从事新兽药研制与开发,E-mail:lnwhj2020@jzmu.edu.cn。
