摘要
为了揭示嗜水气单胞菌的耐药潜力,运用微量肉汤稀释法和比较基因组学方法对其进行了分析。结果显示:恩诺沙星、硫酸新霉素、氟苯尼考、盐酸多西环素对3株菌株有抑制作用,而磺胺间甲氧嘧啶对3株菌株均无抑制作用。菌株的耐药表型未与耐药基因一一对应,某些菌株虽然未注释到耐药基因,也可以表现出相关耐药表型;自测的3株菌株基因组大小差异较大,亲缘关系非近缘,但耐药基因数量基本一致;经ANI(平均核苷酸一致性)计算后,基因组比较分析发现嗜水气单胞菌基因组具有较强的可变性,即使是近缘菌株,其基因组间仍有较大差异;系统发育分析显示,不同宿主来源的菌株在进化树上呈现分散分布,近缘菌株分布在不同国家和地区;所有菌株在CARD(综合抗生素耐药性数据库)中共注释到21种耐药基因,其中7种耐药基因为所有菌株共享。造成这一现象的原因,很可能是由于嗜水气单胞菌基因组较强的可变性以及其宿主分布的广泛性,菌株间进行频繁的基因交流,促进了耐药基因在整个物种的快速扩散,所以即使菌株间基因组变化较大,但耐药基因在菌株间的变化较小。
To reveal the drug-resistance potential of Aeromonas hydrophila,the micro broth dilution method and comparative genomics method were used to analyze its drug-resistance potential in this study.The results showed that enrofloxacin,neomycin sulfate,florfenicol and doxycycline hydrochloride had inhibitory effects on the three strains,while sulfamethoxam had no inhibitory effect on the three strains.The resistance phenotype of the strains was not corresponding to the resistance genes one by one,and some strains could show related resistance phenotype even though the resistance genes were not annotated;The genomes of the three newly sequenced strains differed in size,and their genetic relationships were not close,but the number of drug-resistant genes was basically the same.After calculation of ANI(Average nucleotide identity),comparative analysis of genomes revealed that the genomes of A.hydrophila showed strong variability,and there were still large differences among genomes of even close strains.Phylogenetic analysis showed that the strains from different hosts were scattered in the evolutionary tree,and the related strains were distributed in different countries and regions.A total of 21 drug resistance genes were annotated in all strains in CARD(The Comprehensive Antibiotic Resistance Database),of which 7 were shared by all strains.The reason for this phenomenon is likely to be due to the strong variability of A.hydrophila genome and the wide distribution of its hosts.Frequent gene exchange between strains promotes the rapid spread of drug-resistance genes throughout the species,so even if the genome changes between strains are large,the changes of drugresistance genes between strains are small.
作者
芦运超
邱军强
胡鲲
LU Yunchao;QIU Junqiang;HU Kun(National Demonstration Center for Experimental Fisheries Science Education,Shanghai Ocean University,Shanghai 201306,China;National Pathogen Collection Center for Aquatic Animals,Shanghai Ocean University,Shanghai 201306,China;Key Laboratory of Freshwater Aquatic Genetic Resources,Ministry of Agriculture and Rural Affairs,Shanghai OceanUniversity,Shanghai 201306,China)
出处
《上海海洋大学学报》
CAS
CSCD
北大核心
2023年第6期1216-1223,共8页
Journal of Shanghai Ocean University
基金
国家重点研发计划“蓝色粮仓科技创新”专项课题(2019YFD0900102)
国家淡水水产种质资源库项目(FGRC:18537)。
关键词
嗜水气单胞菌
药敏试验
全基因组测序
比较基因组
系统发育
耐药基因
Aeromonas hydrophila
drug sensitivity test
whole genome sequencing
comparative genome
phylogenetic development
resistance genes
作者简介
芦运超(1994-),男,硕士研究生,研究方向为水生动物医学。E-mail:1687685754@qq.com;通信作者:胡鲲,E-mail:khu@shou.edu.cn。
