摘要
目的探讨不同浓度茯苓三萜对α-葡萄糖苷酶和α-淀粉酶的抑制活性,及其酶促反应动力学特性。方法采用体外α-葡萄糖苷酶和α-淀粉酶抑制活性筛选模型,测定不同浓度的茯苓三萜类对两种酶的抑制作用,采用Lineweaver-Burk双倒数法分析其酶动力学性质。结果体外酶活性抑制试验显示,不同浓度的茯苓三萜类在体外对α-葡萄糖苷酶和α-淀粉酶均有抑制作用,其最大半数抑制浓度(IC_(50))分别为1.40μg/mL、10.43μg/mL,茯苓三萜对α-葡萄糖苷酶的抑制作用为可逆非竞争性抑制,对α-淀粉酶的抑制作用为可逆混合抑制类型。结论茯苓三萜类物质有降血糖活性,且浓度越高抑制效果越明显。本研究可为茯苓降血糖药物的研究和开发提供科学依据。
Objective To investigate the inhibition effects and kinetic properties of Poria cocos triterpenes onα-glucosidase andα-amylase.Methods The inhibitory activities of different concentrations of Poria cocos triterpene onα-glucosidase andα-amylase were tested in vitro,and the kinetic properties were analyzed by Lineweaver-Burk.Results The in vitro enzyme activity inhibition assay showed that different concentrations of Poria triterpenoids inhibited bothα-glucosidase andα-amylase in vitro,and their half-maximum inhibitory concentrations(IC_(50))were 1.40μg/mL and 10.43μg/mL,respectively,and the inhibitory effect of Poria triterpenoids was reversible and non-competitive inhibition onα-glucosidase,and the inhibitory effect onα-amylase was reversible mixed inhibition type.Conclusion The Poria cocos triterpenes have hypoglycemic activity,and show concentration-dependent manner.This study can provide a scientific basis for the research and development of Poria cocos hypoglycemic drugs.
作者
高满军
黄兴琼
莫启贵
陈迷
赵宝清
张丹丹
GAO Man-jun;ZHAO Bao-qing;ZHANG Dan-dan(School of Pharmacy,Xianning Medical College,Hubei University of Science and Technology,Xianning Hubei 437100,China)
出处
《湖北科技学院学报（医学版）》
2023年第6期465-469,共5页
Journal of Hubei University of Science and Technology(Medical Sciences)
基金
糖尿病心脑血管病变湖北省重点实验室开放基金项目(2022TNB05)
湖北科技学院博士科研启动基金(BK202324)。
关键词
茯苓三萜
Α-葡萄糖苷酶
Α-淀粉酶
降血糖
酶动力学
Poria cocos triterpenes
α-Glucosidase
α-Amylase
Blood glucose lowering
Enzyme kinetics
作者简介
通讯作者:赵宝清,E-mail:1280499642@qq.com;通讯作者:张丹丹,18703428460@139.com。
