摘要
转基因及基因编辑的基因修饰植物发展迅猛,相关的检测技术也面临更多挑战,尤其是对于无外源DNA引入的基因编辑产品,其检测难度更高,传统的检测技术已无法满足。基于CRISPR/Cas检测系统中不同Cas蛋白所具有的附属非特异切割活性被广泛应用于分子检测领域,它与LAMP和RPA等温扩增相结合可实现准确快速的现场检测,甚至是对单核苷酸突变的基因编辑产品识别和检测。但是,该检测系统在植物基因修饰产品检测中的应用才刚刚起步,本文对该检测系统的原理及其在基因修饰的植物及其产品中的应用进行了评述。
With the rapid development of genetically modified plants including transgenic and gene-edited plants,the related detection technologies are also facing more challenges,especially for gene editing products without the introduction of exogenous DNA,its detection is more difficult,and the traditional detection technology cannot be satisfied.The accessory nonspecific cleavage activity of different Cas proteins in the CRISPR-Cas-based detection system is widely used in the field of molecular detection.It can be combined with LAMP and RPA isothermal amplification to achieve accurate and rapid on-site detection,even for single gene editing products for nucleotide mutations.However,the application of the detection system in the detection of plant genetically modified products has just started.This paper reviews the principle of the detection system and its application in genetically modified plants and their products.
作者
王渭霞
朱廷恒
赖凤香
万品俊
魏琪
傅强
WANG Weixia;ZHU Tingheng;LAI Fengxiang;WAN Pingjun;WEI Qi;FU Qiang(China National Rice Research Institute/State Key Lab of Rice Biology and Breeding,Hangzhou 310006,China;College of Biotechnology and Bioengineering,Zhejiang University of Technology,Hangzhou 310014,China)
出处
《中国稻米》
北大核心
2023年第6期21-27,共7页
China Rice
基金
中央级公益性科研院所基本科研业务费专项(CPSIBRF-CNRRI-202122)。
作者简介
第一作者:王渭霞,wangweixia@caas.cn;通讯作者:朱廷恒,thzhu@zjut.edu.cn;通讯作者:傅强,fuqiang@caas.cn。
