摘要
以红树莓为研究材料,采用超声辅助乙醇/硫酸铵双水相提取红树莓花色苷。考察了五种因素对红树莓花色苷含量的影响,通过响应面法优化超声辅助双水相萃取技术的最优工艺条件,并进行抗氧化活性分析。结果表明,25%乙醇、0.55 g/mL硫酸铵、超声功率640 W、超声30 min、1∶20的料液比提取效果最好,红树莓花色苷含量为0.219 mg/g。花色苷提取液浓度达到4.8 mg/L时,对DPPH清除率为94.93%,浓度达到5.5 mg/L时,对羟自由基清除率为45.83%,红树莓花色苷清除DPPH和羟自由基的能力远高于作为对照的相同浓度的维生素C溶液,其具有较好的抗氧化活性。
In this experiment,red raspberry(Rubus idaeus L.)was used as the research material,and ul⁃trasonic-assisted ethanol/ammonium sulfate aqueous two-phase extraction from red raspberry anthocya⁃nins was used.We investigated the effects of five factors on the content of anthocyanins in red raspberry flowers,optimized the optimal process conditions of ultrasound assisted aqueous two-phase extraction technology through response surface methodology,and conducted antioxidant activity analysis.The re⁃sults showed that the extraction effect was best with 25%ethanol,0.55 g/mL ammonium sulfate,ultra⁃sound power of 640 W,ultrasound for 30 minutes,and a ratio of 1∶20.The content of anthocyanins in red raspberry was 0.219 mg/g.When the concentration of anthocyanin extract reaches 4.8 mg/L,the DPPH clearance rate is 94.93%,and when the concentration reaches 5.5 mg/L,the hydroxyl radical clearance rate is 45.83%.The ability of red raspberry anthocyanin to clear DPPH and hydroxyl radicals is much higher than that of the same concentration of vitamin C solution as the control,and it has good antioxidant activity.
作者
杨晓娜
陈宏艳
陈自宏
YANG Xiaona;CHEN Hongyan;CHEN Zi-hong(School of Resources and Environment,Baoshan University,Baoshan Yunnan 678000,China)
出处
《保山学院学报》
2023年第5期7-16,共10页
JOURNAL OF BAOSHAN UNIVERSITY
基金
国家自然科学基金项目“中国西南高黎贡山虫生真菌地理分布格局及驱动机制”(项目编号:32060638)
云南省中青年学术和技术带头人后备人才项目(项目编号:202205AC160061)
云南省教育厅高黎贡山虫生真菌资源保护与绿色发展重点实验室建设项目。
关键词
红树莓
双水相提取
超声提取
花色苷
抗氧化活性
Rubus idaeus L.
Aqueous two-phase extraction
Ultrasonic extraction
Anthocyanins
Anti⁃oxidant activity
作者简介
第一作者:杨晓娜(1984-),女,云南临沧人,硕士,讲师,研究方向为天然产物研究与开发;通信作者:陈自宏(1978-),男,云南陆良人,博士,教授,研究方向为虫生真菌研究。
