摘要
目的:探究赛艇测功仪2000 m全力划过程中不同分段的血液代谢组学特征,分析不同分段与供能相关的潜在生物标志物。方法:选取12名男子赛艇运动员(一级运动员)在4天内依次进行赛艇测功仪2000 m全力划及过程中500、1000和1500 m模拟划测试,采集测试前安静状态和每次运动后即刻的肘静脉血样(分别标记为A、B、C、D和E组),基于高效化学同位素标记-液质联用(LC-MS)代谢组学技术进行分析。采用IsoMS Pro 1.2.15软件进行多元统计分析(PCA、PLS-DA)和火山图分析遴选出差异代谢物。差异代谢物通过MetaboAnalyst 5.0平台进行层次聚类分析,并结合Nova MT代谢组学数据库与HMDB在线数据库来注释潜在生物标志物和评估它们的生物学作用。结果:与A组(安静)相比,C组(500 m)运动后即刻的L-赖氨酸、鸟氨酸、丝氨酸、甘氨酸、4-羟脯氨酸和胱氨酸显著减少(P<0.01),N-甲基-L-谷氨酸、泛酸、对羟基苯乙酰甘氨酸、肌酐和腺嘌呤显著增加(P<0.01);与C组(500 m)相比,D组(1000 m)运动后即刻的4-羟脯氨酸、甘氨酸、四氢嘧啶、L-缬氨酸和5-氨基戊酸显著减少(P<0.01),泛酸、抗坏血酸、对羟基苯乙酰甘氨酸、次黄嘌呤、磷酸乙醇胺、3-亚砜-L-丙氨酸、N-甲基-L-谷氨酸、胱氨酸、高香草酸和腺嘌呤显著增加(P<0.01);与D组(1000 m)相比,E组(1500 m)运动后即刻的吲哚-3-羧酸显著减少(P<0.01),次黄嘌呤和抗坏血酸显著增加(P<0.01);与E组(1500 m)相比,B组(2000 m)运动后即刻的茶碱显著减少(P<0.01),4-羟脯氨酸、甘氨酸、次黄嘌呤、抗坏血酸、氨基己二酸和甲硫氨酸显著增加(P<0.01)。结论:有氧代谢、无氧代谢和氧化应激相关的差异代谢物显著影响赛艇测功仪2000 m全力划过程中不同分段的供能和运动表现,其中,泛酸、N-甲基-L-谷氨酸、腺嘌呤和次黄嘌呤等无氧代谢产物在0~500 m分段或1500~2000 m分段发生显著变化,可作为鉴别赛艇运动员无氧供能的潜在生物标志物。
Objective To explore the blood metabonomic characteristics of different segments during the 2000 m all-out rowing ergometer process and analyze the potential biomarkers related to energy supply in different segments.Methods Twelve national first-grade rowing athletes were selected to under⁃go the 2000 m all-out rowing ergometer test within 4 days,with the simulated 500,1000 and 1500 m rowing tests carried out during the process.Venous blood samples were collected from the elbow im⁃mediately before and after the exercise,and analyzed using the high-efficiency chemical isotope label⁃ing liquid chromatography-mass spectrometry(LC-MS)metabolomic technology.Moreover,differential metabolites were selected according to the multivariate statistical analysis(PCA,PLS-DA)and volcano plot analysis using the IsoMS Pro 1.2.15 software.Differential metabolites were clustered hierarchically using the MetaboAnalyst 5.0 platform,and annotated with potential biomarkers and their biological roles by consulting the Nova MT metabolomics database and HMDB online database.Results Compared to at rest,the levels of L-lysine,ornithine,serine,glycine,4-hydroxyproline,and cysteine de⁃creased significantly after 500 m exercise(P<0.01),while those of N-methyl-L-glutamate,pantothenic acid,p-hydroxyphenylacetyl glycine,creatinine,and adenine significantly increased(P<0.01)immediate⁃ly.Moreover,compared to after 500 m test,the levels of 4-hydroxyproline,glycine,tetrahydropyrimi⁃dine,L-valine,and 5-aminopentanoic acid decreased significantly(P<0.01),while those of pantothen⁃ic acid,ascorbic acid,p-hydroxyphenylacetyl glycine,xanthine,phosphoethanolamine,3-sulfo-L-ala⁃nine,N-methyl-L-glutamate,cysteine,vanillic acid,and adenine increased significantly(P<0.01)im⁃mediately after 1000 m exercise.Compared to after 1000 m test,there was a significant decrease in the level of indole-3-carboxylic acid(P<0.01),but a significant increase in that of xanthine and ascor⁃bic acid(P<0.01)immediately after 1500 m test.However,compared with after the 1500 m test,the level of theophylline decreased significantly(P<0.01),while that of 4-hydroxyproline,glycine,xan⁃thine,ascorbic acid,aminocaproic acid,and methionine significantly increased(P<0.01)immediately af⁃ter the test of 2000 m.Conclusion Differential metabolites related to aerobic metabolism,anaerobic me⁃tabolism,and oxidative stress have a significant effect on the energy supply and athletic performance in different segments of the 2000 m all-out rowing ergometer process.Such anaerobic metabolism prod⁃uctsas pantothenic acid,N-methyl-L-glutamate,adenine,and xanthine,show significant changes in the 0-500 m and 1500-2000 m segments and can serve as potential biomarkers for identifying anaero⁃bic energy supply of rowing athletes.
作者
林家仕
姚安
蒋国用
元曹安
陈建明
王向党
秦宇飞
Lin Jiashi;Yao An;Jiang Guoyong;Yuan Caoan;Chen Jianming;Wang Xiangdang;Qing Yufei(College of Physical Education,Jimei University,Xiamen 361021,China;Beijing Research Institute of Sports Science,Beijing 100075,China)
出处
《中国运动医学杂志》
CAS
CSCD
北大核心
2023年第7期527-539,共13页
Chinese Journal of Sports Medicine
基金
国家重点研发计划“科技冬奥”重点专项(2018YFF0300403)
教育部人文社科研究项目(20YJA890014)。
作者简介
第1作者:林家仕,Email:linjiashi1980@126.com;通信作者:陈建明,Email:jmchen2@126.com。
