摘要
对兰州百合采用热水提取和不同体积分数乙醇(30%、50%、70%、80%)醇沉得到4种多糖,分别记为BLP-30、BLP-50、BLP-70和BLP-80。对上述4种多糖性能进行了测试,比较了多糖对α-淀粉酶和α-葡萄糖苷酶的抑制能力,分析了多糖基本组成与降血糖活性的相关性。结果表明,4种多糖均不含或含有极少量糖醛酸和蛋白,BLP-80可溶性总糖质量分数最高,为30.96%。4种多糖为混合多糖,均由甘露糖、葡萄糖和少量半乳糖组成,重均相对分子质量从BLP-30-1的625k Da逐渐减至BLP-70-4的2.45kDa。其中,BLP-30和BLP-50为葡聚糖,BLP-70为半乳甘露葡聚糖,BLP-80为甘露葡聚糖,BLP-30中甘露糖、葡萄糖、半乳糖摩尔分数分别为10.18%、88.67%、1.15%;BLP-50中上述3种多糖摩尔分数分别为2.49%、97.02%、0.49%;BLP-70中上述3种多糖摩尔分数分别为26.94%、68.21%、4.85%;BLP-80中甘露糖和葡萄糖摩尔分数分别为41.56%和58.44%。4种多糖对α-淀粉酶和α-葡萄糖苷酶的抑制能力强弱顺序分别为BLP-70>BLP-80>BLP-30>BLP-50和BLP-50>BLP-70>BLP-80>BLP-30。4种多糖对α-淀粉酶的抑制能力与甘露糖和半乳糖的摩尔分数呈正相关,对α-淀粉酶的抑制能力与葡萄糖的摩尔分数呈负相关;对α-葡萄糖苷酶的抑制能力与可溶性总糖质量分数、糖醛酸质量分数、甘露糖摩尔分数呈负相关;对α-葡萄糖苷酶的抑制能力与葡萄糖摩尔分数呈正相关。
Four polysaccharides,named BLP-30,BLP-50,BLP-70 and BLP-80,respectively,were obtained from hot water extraction and alcohol precipitation with different volume fractions of ethanol(30%,50%,70%and 80%).The properties of the four polysaccharides were then tested,with their inhibitory ability onα-amylase andα-glucosidase compared and the correlation between the basic composition and their hypoglycemic activity analyzed.The results showed that all the four polysaccharides contained little or no uronic acid and protein,and BLP-80 had the highest amount of total soluble sugar(30.96%).All the four polysaccharides were mixed polysaccharides,with BLP-30 and BLP-50 glucans,BLP-70 galactomannan glucans and BLP-80 mannoglucan glucans,consisting of mannose,glucose and a small amount of galactose,while the mass average relative molecular mass gradually decreased from 625 kDa for BLP-30-1 to 2.45 kDa for BLP-70-4.The molar fractions of mannose,glucose and galactose in BLP-30 were 10.18%,88.67%and 1.15%,respectively,in BLP-50 were 2.49%,97.02%and 0.49%,respectively,and in BLP-70 were 26.94%,68.21%and 4.85%,respectively.The mole fractions of mannose and glucose in BLP-80 were 41.56%and 58.44%,respectively.The inhibitory effects of the four polysaccharides onα-amylase andα-glucosidase gradually decreased in the following order:BLP-70>BLP-80>BLP-30>BLP-50 and BLP-50>BLP-70>BLP-80>BLP-30,respectively.The inhibitory ability of the four polysaccharides onα-amylase was positively correlated with the molar fraction of mannose and galactose,that of the polysaccharides onα-amylase was negatively correlated with the molar fraction of glucose,that of the polysaccharides onα-glucosidase was negatively correlated with the mass fraction of total soluble sugars and uronic acid,and the molar fraction of mannose,and that of polysaccharides onα-glucosidase was positively correlated with the molar fraction of glucose.
作者
张心驰
惠和平
郭栋费
魏赛雪
郭敏
李晓东
ZHANG Xinchi;HUI Heping;GUO Dongfei;WEI Saixue;GUO Min;LI Xiaodong(College of Pharmacy,Gansu University of Chinese Medicine,Lanzhou 730010,Gansu,China;School of Biomedicine and Food Engineering,Shangluo University,Shangluo 726000,Shaanxi,China;Gansu Provincial Hospital of Traditional Chinese Medicine,Lanzhou 730050,Gansu,China;Professional Technical Center for Secondary Exploitation and Clinical Evaluation of Longyao Large Varieties,Gansu Academy of Traditional Chinese Medicine,Lanzhou 730050,Gansu,China)
出处
《精细化工》
EI
CAS
CSCD
北大核心
2023年第10期2231-2240,共10页
Fine Chemicals
基金
国家自然科学基金(82160818)
甘肃省高等学校创新基金(2021A-260)。
关键词
梯度醇沉
兰州百合多糖
结构表征
降血糖活性
相关性分析
中药现代化技术
gradient alcohol precipitation
Lanzhou lily polysaccharides
structural characterization
hypoglycemic activity
correlation analysis
modernization technology of traditional Chinese medicines
作者简介
张心驰(1997—),男,硕士生,E-mail:517354206@qq.com;联系人:惠和平(1982—),男,博士,副教授,E-mail:lzdxhui@163.com;联系人:李晓东(1979—),男,博士,副研究员,E-mail:li_xd2005@126.com。
