摘要
目的:考察清心莲子饮提取物对大鼠肾炎的治疗作用和安全性。方法:采用纸片扩散法和二倍浓度稀释法,检测清心莲子饮提取物对金黄色葡萄球菌、大肠埃希菌和铜绿假单胞菌的体外抑菌活性;采用大鼠细菌性肾盂肾炎模型、阿霉素诱导的大鼠肾小球肾炎模型和大鼠IgA肾病模型,评价清心莲子饮提取物对大鼠肾炎的治疗作用;采用小鼠急性毒性实验,初步评价清心莲子饮提取物的安全性。结果:清心莲子饮提取物对3株致病菌无明显体外抑菌活性,但在体内可明显降低肾盂肾炎大鼠血清肌酐和尿素氮含量、显著减少大鼠尿潜血和尿蛋白程度;清心莲子饮提取物8 g·kg^(-1)剂量可显著降低阿霉素肾炎大鼠血清肌酐、尿素氮水平,但对大鼠肾脏指数和24 h尿蛋白量无明显影响;清心莲子饮提取物8 g·kg^(-1)剂量可明显降低IgA肾病大鼠24 h尿蛋白量,但对大鼠血清肌酐、尿素氮水平、肾脏指数均无明显影响。小鼠急性毒性实验结果显示:清心莲子饮提取物的最大给药量为48 g·kg^(-1),在该剂量下未见明显毒性反应和死亡情况。结论:清心莲子饮提取物对3种大鼠实验性肾炎具有治疗作用。
Objective:To investigate the therapeutic effect and safety of the extract of Qingxin Lianzi Decoction(QLD)on nephritis in rats.Methods:The antibacterial activity of QLD extract against Staphylococcus aureus,Escherichia coli and Pseudomonas aeruginosa in vitro was determined by disk diffusion and double dilution methods.Pyelonephritis model induced by bacterial,adriamycin⁃induced glomerulonephritis model and IgA nephropathy model in rats were used to evaluate the therapeutic effect on nephritis in rats.The safety of QLD extract was preliminarily evaluated using the acute toxicity test in mice.Results:QLD extract had no obvious antibacterial activity against the three pathogenic bacteria in vitro,but it significantly reduced the contents of creatinine and urea nitrogen in serum,decreased the levels of urinary occult blood and urine protein in pyelonephritis rats.At the same time,the 8 g·kg^(-1)dose of QLD significantly reduced the contents of creatinine and urea nitrogen in serum,but had no significant effect on the kidney index and urine protein in glomerulonephritis rats.Moreover,the 8 g·kg^(-1)dose of QLD significantly reduced the level of urine protein in IgA nephropathy rats,but had no significant effect on creatinine,urea nitrogen and kidney index.The results of acute toxicity showed that the maximum dose of QLD extract is 48 g·kg^(-1),and no obvious toxicity and death were observed.Conclusion:QLD extract has therapeutic effects on three experimental nephritis in rats.
作者
吴德松
柴琇瑛
颜宏
赵道强
郭琰
李婷
WU De-song;CHAI Xiu-ying;YAN Hong;ZHAO Dao-qiang;GUO Yan;LI Ting(Yunnan Institute of Materia Medica,Kunming 650111,China;Yunnan Province Company Key Laboratory for TCM and Ethnic Drug of New Drug Creation,Kunming 650111,China)
出处
《中国新药杂志》
CAS
CSCD
北大核心
2023年第17期1774-1782,共9页
Chinese Journal of New Drugs
基金
昆明大健康科技创新中心资助项目(2019⁃1⁃N⁃25318000003565)。
关键词
经典名方
清心莲子饮
抗菌
肾盂肾炎
肾小球肾炎
classic prescription
Qingxin Lianzi Decoction
antibacterial
pyelonephritis
glomerulus nephritis
作者简介
吴德松,男,硕士,高级工程师,主要从事天然药物研究、开发工作。E⁃mail:19785025@qq.com;通讯作者:李婷,女,硕士,工程师,主要从事天然药物药效学评价工作。E⁃mail:1091607276@qq.com。
