摘要
α-乳香酸(α-BA)是乳香药材经过提取、分离、纯化后的产物,可用于治疗多种炎症性疾病。本研究旨在通过体内外实验评估α-BA在改善缺血再灌注损伤诱导的急性肾损伤中的作用。对大鼠进行右肾切除和左肾缺血再灌注损伤,损伤前0.5 h给予α-BA处理。体内实验,对组织进行肾功能、组织病理学和TUNEL染色分析。体外实验,对近端肾小管细胞(HK-2)进行氧糖剥夺(OGD)操作,以模仿缺血再灌注损伤,并检测细胞存活率、免疫荧光染色及Toll样受体4(TLR4)和核因子(NF)-κB表达。结果显示,给予α-BA的模型组大鼠血清肌酐和血尿素氮水平显著降低,并缓解细胞凋亡和肾脏形态学改变;经α-BA处理的HK-2细胞,NF-κBp6的表达明显降低。表明α-BA改善缺血再灌注损伤诱导的急性肾损伤,部分原因是通过TLR4介导的炎症通路促进肾小管细胞存活,从而改善肾功能。
α-Boswellic acid(α-BA)is a bioactive compound derived from the resin of the African myrrh tree,which has been shown to have therapeutic potential for a range of inflammatory disorders.In the present study,we aimed to evaluate the effects ofα-BA on improving ischemia-reperfusion injury(IRI)-induced acute kidney injury(AKI),both in vivo and in vitro.In this experiment,rats that underwent right nephrectomy and left kidney IRI were administered withα-BA 30 min before IRI.The rats’renal function was evaluated,and their kidney tissues were subjected to histopathological analysis and TUNEL staining.For in vitro evaluation,the proximal renal tubular cells(HK-2)were subjected to oxygen-glucose deprivation(OGD).Cell viability,immunofluorescence staining,and Toll-like receptor 4(TLR4)and nuclear factor(NF)-κB expressions were investigated.After treatment withα-BA,the levels of serum creatinine and blood urea nitrogen were significantly reduced in the rats subjected to IRI,and apoptosis was alleviated,along with improvements in renal morphological changes.Moreover,the treatment of HK-2 cells withα-BA effectively reduced the expression of NF-κB p65.Collectively,the results suggested thatα-BA improved IRI-induced AKI in part by promoting the survival of renal tubular cells through the TLR4-mediated inflammatory pathway,thereby improving renal function.
作者
王番
李锐莉
王文军
周晓燕
刘美佑
赵瑾怡
文爱东
王婧雯
贾艳艳
Fan Wang;Ruili Li;Wenjun Wang;Xiaoyan Zhou;Meiyou Liu;Jinyi Zhao;Aidong Wen;Jingwen Wang;Yanyan Jia(Xijing Hospital,Medical University of the Air Force,Department of Pharmacy,710032,Xi’an,Shaanxi,China)
基金
The National Natural Science Foundation of China(Grant No.81703795)
the Traditional Chinese Medicine Research Project of Shaanxi Province Traditional Chinese Medicine Administration(Grant No.JCMS050)
the Shaanxi Province Natural Science Basic Research Project(Grant No.2018JQ8065)
the Shaanxi Provincial Key R&D Program Project(Grant No.2020ZDLSF03-09)。
关键词
急性肾损伤
缺血再灌注损伤
炎症
凋亡
α-乳香酸
Acute kidney injury
Ischemia reperfusion injury
Inflammation
Apoptosis
α-Boswellic acid
作者简介
王番,contributed equally to this study;李锐莉,contributed equally to this study;王文军,contributed equally to this study;周晓燕,contributed equally to this study;Corresponding author:贾艳艳,Tel.:+86-29-84771794,E-mail:25961754@qq.com;Corresponding author:王婧雯,wangjingwen8021@163.com。
