摘要
本文旨在探究添加褐藻多酚提取物对奶牛瘤胃体外发酵和主要瘤胃细菌含量的影响。以水稻(Oryza sativa L.)秸秆∶豆(Glycine max)粕=6∶4为发酵底物,试验共分9组,前4组在底物的基础上分别添加0,125,250,500μg·mL^(-1)褐藻多酚(Phlorotannin,PT);后4组在前4组的基础上添加30 mg聚乙二醇(PEG)。结果表明:褐藻多酚提取物的添加能够显著降低瘤胃pH值和氨态氮浓度(P<0.05),促进瘤胃发酵类型的转变,显著提高了普雷沃氏菌和牛链球菌含量,降低瘤胃甲烷短杆菌的含量(P<0.05);对瘤胃发酵各项参数进行综合评定,无论是否添加PEG,125μg·mL^(-1)组的多项组合效应值(MFAEI)均最高。综上所述,日粮中添加褐藻多酚提取物为瘤胃发酵提供了一个更具酸性的环境,减少了瘤胃内蛋白质的分解,具有降低甲烷排放的潜能,且在本试验中褐藻多酚的最佳添加量为125μg·mL^(-1)。
This study aimed to investigate the effects of Phlorotannins extract on in vitro rumen fermentation and main rumen bacteria in dairy cows.The experiment used rice straw:soybean meal(6∶4)as a fermentation substrate apportioned into eight treatment groups,namely 0,125,250,and 500μg·mL^(-1) phlorotannin(PT)supplements and 30 mg polyethylene glycol(PEG)supplementation to the all individual phlorotannin(PT)supplements.The results showed that:The addition of phlorotannins extract significantly decreased pH value and ammonia nitrogen concentration(P<0.05)in rumen substances,promoted the transformation of rumen fermentation type,increased Prevotella ruminicola and Streptococcus bovis,and decreased Methanobrevibacter ruminantium(P<0.05).The MFAEI of 125μg·mL^(-1) supplement was the highest in all rumen fermentation parameters,regardless of PEG addition or not.In conclusion,dietary supplement of phlorotannins extract provided a more acidic environment for rumen fermentation,consequently to reduce the protein decomposition,and potentially decrease methane emissions.In this experiment,the optimal dosage of phlorotannins supplement was 125μg·mL^(-1).
作者
陈誉华
魏元浩
吉慧敏
黄倩倩
CHEN Yu-hua;WEI Yuan-hao;JI Hui-min;HUANG Qian-qian(Yangzhou University,Yangzhou,Jiangsu Province 225000,China)
出处
《草地学报》
CAS
CSCD
北大核心
2023年第8期2343-2351,共9页
Acta Agrestia Sinica
基金
财政部和农业农村部:国家现代农业产业技术体系(CARS36)
国家自然科学基金(31902191)共同资助。
关键词
褐藻多酚
瘤胃发酵
干物质降解率
瘤胃细菌
Phlorotannins
Rumen fermentation
Dry matter degradation rate
Rumen bacteria
作者简介
陈誉华(1996-),女,汉族,江苏南通人,硕士研究生,从事植物提取物的开发与利用研究,E-mail:Ovaltine0930@163.com;通信作者:黄倩倩,E-mail:huangq0315@126.com。
