摘要
目的:基于谱效关系研究,寻找景天三七抗氧化活性的物质基础,为其质量控制提供一定的参考依据。方法:建立不同产地景天三七的HPLC-ECD(High performance liquid chromatography-Electrochemical Detection,HPLC-ECD)指纹图谱并进行相似度评价;通过偏最小二乘回归分析和灰色关联分析研究指纹图谱与其抗氧化活性之间的谱效关系。结果:建立了景天三七的HPLC-ECD指纹图谱,样品间的相似度较高,共标定17个共有峰,通过与对照品比对鉴别出了没食子酸、原儿茶酸、色氨酸、没食子酸甲酯、表没食子儿茶素、咖啡酸、表没食子儿茶素没食子酸酯、鸢尾酚酮、杨梅苷、异槲皮苷、迷迭香酸和槲皮苷等12种成分;谱效关系表明,没食子酸、原儿茶酸、表没食子儿茶素、表没食子儿茶素没食子酸酯、迷迭香酸和槲皮苷的VIP值均大于1;其中没食子酸、迷迭香酸和槲皮苷的关联度接近于0.8。分子对接结果表明,表没食子儿茶素没食子酸酯、杨梅苷、异槲皮苷、迷迭香酸和槲皮苷的Libdock Score较高。因此,表没食子儿茶素没食子酸酯、杨梅苷、异槲皮苷、迷迭香酸和槲皮苷等化合物为景天三七抗氧化活性的关键成分。结论:结合化学计量学方法进行谱效关系研究,确定了景天三七抗氧化活性物质基础,可为景天三七的质量评价提供了一定的科学依据。
Objective:To find the material basis of antioxidant activity of Sedum aizoon L.based on the study of spectrum-effect relationship,which provided a certain reference for its quality control.Method:The fringerprint of Sedum aizoon L.from different areas were established by high performance liquid chromatography-electrochemical detection(HPLC-ECD)and the similarity of the samples were evaluated.At the same time,the spectrum-effect relationship between HPLC-ECD fingerprint of Sedum aizoon L.and antioxidant activity was studied by partial least squares regression analysis and grey correlation analysis.Results:The HPLC-ECD fingerprint of Sedum aizoon L was established.The similarity of the samples was high and 17 common peaks were obtained.Among them,the 12 components were identified with the standard solution,including gallic acid,protocatechuic acid,tryptophan,methyl gallate,epigallocatechin,caffeic acid,epigallocatechin gallate,iriflophene,myricitrin,isoquercitrin,rosmarinic acid and quercitrin.The spectrum relationship showed that the variables important in projection(VIP)scores of gallic acid,protocatechuic acid,epigallocatechin,epigallocatechin gallate,rosmarinic acid and quercitrin were greater than 1.Among them,the grey correlation degree of gallic acid,rosmarinic acid and quercitrin was nearly 0.8.Furthermore,epigallocatechin gallate,myricitrin,isoquercitrin,rosmarinic acid and quercitrin had higher libdock score in molecular docking.Therefore,epigallocatechin gallate,myricitrin,isoquercitrin,rosmarinic acid and quercitrin were the primary components contributing to the antioxidant activity of Sedum aizoon L..Conclusion:The basis of antioxidant activity of Sedum aizoon L.was determined by the study of spectrum effect relationship with chemometrics,which provides a certain scientific basis for the quality evaluation of Sedum aizoon L..
作者
杨锡金
王艳
陈梓瀚
刘明芸
吴俊
向杨
陈荣祥
周亚平
YANG Xijin;WANG Yan;CHEN Zihan;LIU Mingyun;WU Jun;XIANG Yang;CHEN Rongxiang;ZHOU Yaping(School of Pharmacy,Zunyi Medical University,Zunyi 563000,China;School of Basic Medicine,Zunyi Medical University,Zunyi 563000,China)
出处
《食品工业科技》
CAS
北大核心
2023年第16期15-24,共10页
Science and Technology of Food Industry
基金
国家自然科学基金(81960707,81760652)
遵义医科大学大学生创新创业项目(ZYDC2021065,ZYDC2021152)。
关键词
景天三七
高效液相色谱指纹图谱
化学计量学
抗氧化活性
谱效关系
Sedum aizoon L.
high performance liquid chromatography fingerprint
chemometrics
antioxidant activity
spectrum effect relationship
作者简介
杨锡金(1998−),女,硕士,研究方向:药物分析,E-mail:yxj505507403@163.com;通信作者:陈荣祥(1984−),男,博士,副教授,研究方向:药用植物色谱分析,E-mail:chenrongxiang2014@163.com;通信作者:周亚平(1986−),女,博士,副教授,研究方向:药物分析,E-mail:yaping20302@163.com。
