摘要
目的:建立高效液相色谱-电化学检测法(High performance liquid chromatography-electrochemical detection,HPLC-ECD)测定鱼腥草叶中多酚类化合物,并采用相关性分析方法明确不同多酚单体化合物对于抗氧化活性的贡献。方法:鱼腥草叶经80%(v/v)甲醇超声提取,基于建立的HPLC-ECD检测方法测定12种多酚化合物含量。采用福林酚法测定鱼腥草叶总酚含量,以自由基清除能力和铁离子还原能力进行抗氧化活性评价,采用聚类热图和灰色关联分析法进行多元统计分析。结果:建立的HPLC-ECD方法线性、精密度、重复性、稳定性均良好,平均加样回收率为93.25%~103.97%,RSD为1.99%~4.47%。不同来源的鱼腥草叶的总酚含量为5.98~52.86 mg GAE/g,其含量与ABTS+、DPPH自由基清除能力和铁离子还原能力显著性相关(P<0.05)。鱼腥草中含量较高的多酚类化合物为咖啡酸和槲皮素的衍生物,12种酚类化合物均与抗氧化活性有较高的关联度,其中异槲皮苷、槲皮苷、异绿原酸A、金丝桃苷、芦丁的相关性最高。本研究明确了鱼腥草叶抗氧化的主要活性成分,可以为鱼腥草质量评价和资源开发提供参考。
Objective:To establish a high performance liquid chromatography-electrochemical detection(HPLC-ECD)method for the determination of polyphenolic compounds in the leaves of Houttuynia cordata and investigate the contribution of different compounds to the antioxidant activity by the correlation analysis.Methods:The leaves of Houttuynia cordata were extracted by ultrasonic extraction with 80%(v/v)methanol,and the content of 12 polyphenolic compounds was determined by the established HPLC-ECD method.The total phenolics content of Houttuynia cordata leaves was determined by the Folin-ciocalteu method.The antioxidant activities were evaluated by radical scavenging ability and ferric reducing antioxidant power(FRAP).Cluster heat map and gray correlation analysis were used for multivariate statistical analysis.Results:The established HPLC-ECD method showed good linearity,precision,repeatability and stability,with an average recovery of 93.25%~103.97%and RSD value of 1.99%~4.47%.The total phenolic content of Houttuynia cordata leaves from different sources ranged from 5.98~52.86 mg GAE/g,which was significantly correlated with ABTS+,DPPH radical scavenging ability and ferric ion reduction ability(P<0.05).The polyphenolic compounds with high content in Houttuynia cordata were derivatives of caffeic acid and quercetin.All of the 12 phenolic compounds had a high degree of correlation with antioxidant activity,among which isoquercitrin,quercetin,isochlorogenic acid A,hyperoside and rutin were the highest.The present study elucidated the primary antioxidant components in Houttuynia cordata leaves,which would provide reference for the quality evaluation and resource development of Houttuynia cordata.
作者
雷娇
邵起菊
肖欣
李志荣
洪金伶
王森
陈荣祥
LEI Jiao;SHAO Qiju;XIAO Xin;LI Zhirong;HONG Jinling;WANG Sen;CHEN Rongxiang(School of Pharmacy,Zunyi Medical University,Zunyi 563000,China;School of Basic Medicine,Zunyi Medical University,Zunyi 563000,China;Zunyi Engineering Technology Research Center of Physical and Chemical Analysis,Zunyi 563000,China)
出处
《食品工业科技》
CAS
北大核心
2024年第4期221-228,共8页
Science and Technology of Food Industry
基金
国家自然科学基金(81760652)
遵义市科技支撑项目(遵市科合HZ(2020)67号)
遵义医科大学2019年度学术新苗培养及创新探索专项项目(黔科平台人才[2019]-006)
遵义医科大学大学生创新创业项目(ZYDC2022035)。
关键词
鱼腥草叶
含量测定
抗氧化活性
多酚
高效液相色谱法
Houttuynia cordata leaf
content determination
antioxidant activity
polyphenols
high performance liquid chromatography
作者简介
雷娇(1998−),女,硕士研究生,研究方向:食品药品分析,E-mail:1561676965@qq.com;通信作者:王森(1978−),男,博士,教授,研究方向:中药药剂分析,E-mail:wangsen912912@126.com;通信作者:陈荣祥(1984−),男,博士,副教授,研究方向:食品药品分析,E-mail:chenrongxiang2014@163.com。
