摘要
研究并筛选适合纯化黄花菜多酚的大孔树脂,优化其分离纯化工艺。通过紫外分光光度法测定黄花菜中总多酚含量,对比7种大孔树脂对黄花菜总多酚的静态吸附量、解吸量,筛选出最佳树脂,并通过单因素实验优化纯化工艺。结果表明:HPD-600大孔树脂为最适宜纯化黄花菜多酚树脂,其静态吸附、解吸最佳条件为上样液质量浓度0.125 mg·mL^(-1)、pH=6、吸附时间3 h,采用pH为7的60%乙醇进行解吸;HPD-600大孔树脂吸附黄花菜多酚过程符合准一级动力学方程,吸附热力学更符合Langmuir方程。动态吸附-解吸最佳工艺条件为:上样流速2.4 BV·h^(-1)、上样量180 mL、洗脱流速2.4 BV·h^(-1)、乙醇洗脱用量100 mL。在此条件下可将黄花菜多酚纯度从9.42%提高至75.64%,说明HPD-600型树脂可作为黄花菜多酚分离纯化的介质。
The purification process of total polyphenols from Hemerocallis citrina by macroporous adsorption resins were optimized.Ultraviolet-visible(UV-Vis)spectrophotometry was employed to detect the content of total polyphenols in Hemerocallis citrina.Seven different macroporous adsorption resins were used for the purification,and the optimum one was selected based on the absorption rate and desorption rate of total polyphenols in Hemerocallis citrina.The results showed that HPD-600 resin was suitable for the purification of polyphenols from Hemerocallis citrina.The optimum conditions were as follows:sample mass concentration 0.125 mg·mL^(-1),the sample solution pH value 6.0,the adsorption time 3 h.The adsorption process was consistent with the pseudo-first-order model,Langmuir model could accurately describe the adsorption behavior of HPD600 macroporous resin;The desorption process conditions were that the ethanol volume fraction was 60%,the pH value was 7.0.The conditions of dynamic adsorption-desorption process were flow rate at 2.4 BV·h^(-1) and sample loading of 180 mL,100 mL volume fraction60%ethanol as elution with velocity at 2.4 BV·h^(-1).Under the conditions,the purity of polyphenols from Hemerocallis citrinas could reach 75.64%.Therefore,HPD-600 resin was suitable for separation and purification of polyphenols from Hemerocallis citrin.
作者
张腊腊
王玉峰
胡浩斌
韩明虎
ZHANG La-la;WANG Yu-feng;HU Hao-bin;HAN Ming-hu(College of Chemistry and Chemical Engineering,Longdong University,Qingyang Gansu 745000,China)
出处
《当代化工》
CAS
2023年第6期1345-1349,1400,共6页
Contemporary Chemical Industry
基金
甘肃省科技厅青年基金项目(项目编号:21JR7RM193)
庆阳市自然基金项目(项目编号:QY2021A-F012)。
关键词
黄花菜
总多酚
大孔树脂
纯化
纯度
Hemerocallis citrina
Total polyphenols
Macroporous resins
Purification
Purity
作者简介
张腊腊(1986-),女,甘肃省庆城县人,副教授,硕士研究生,2012年毕业与兰州交通大学化学工艺专业,研究方向:精细化工。E-mail:1029057094@qq.com。
