摘要
在水温(28.5±1.0)℃下,将初始平均体质量(45.00±3.00)g的锦鲤(Cyprinus carpio)放入780 mm×575mm×467 mm(实际加水110 L)的养殖箱中,采用槟榔(Areca catechu L.)和青蒿(Artemisia carvifolia)1∶1混合制剂粉碎后无纺布挂袋药浴,浸泡浓度分别为0 mg·L^(-1)(对照组)、6.25 mg·L^(-1)(A组)、12.5 mg·L^(-1)(B组)、125mg·L^(-1)(C组)和250 mg·L^(-1)(D组)。试验开始的第0 d、3 d、6 d、9 d、12 d和15 d随机取出3尾鱼测定血清中C3a、C5aR、PGE2、PGF-2a、LZM和IL-6含量及鳃部表观变化,研究这两种中草药浸泡对锦鲤非特异性免疫因子活性、鳃及鳃丝组织病变的影响。结果表明,同一天中,浸泡组C3a、PGE2、PGF-2a和LZM含量显著上升(P<0.05),C5aR和IL-6含量无显著变化(P>0.05);同浓度的C3a、C5aR、PGE2、PGF-2a、LZM和IL-6的含量随浸泡时间延长先升高后降低,其中D组中含量变化最大。切片显示,在试验第0 d,鱼的鳃丝正常,无病变。在试验第9 d,各浸泡组鳃丝末端均无血细胞增多导致的膨大现象,炎症细胞未增多;而对照组鳃丝红肿溃烂。由此可见,此中药配方在适量范围内可以调节锦鲤体内的炎性因子,增强非特异性免疫。浸泡浓度250 mg·L^(-1)组效果较好。
Koi(Cyprinus carpio)with an initial average body mass of(45.00±3.00)g were placed in a breeding box of 780 mm×575 mm×467 mm(actually added water 110 L)and bathed the water with a non-woven bag of Chinese herbal medicine Areca catechu and Artemisia carvifolia at ratio of 1:1 preparations at water temperature of(28.5±1.0)°C at concentrations of 0 mg·L^(-1)(control group)and 6.25 mg·L^(-1)(group A),12.5 mg·L^(-1)(group B),125 mg·L^(-1)(group C)and 250 mg·L^(-1)(group D).The contents of C3a,C5aR,PGE2,PGF-2a,LZM and IL-6 were measured in serum of 3 fish samples collected randomly and the appearance of gills and lesions of gill filaments were observed on the 0th,3rd,6th,9th,12th and 15th day of the experiment to effects of Chinese herbal medicine bath on the activity of non-specific immune factors and lesions of gill and gill filament tissues in koi carp.The contents of C3a,PGE2,PGF-2a and LZM were shown to be increased significantly in the soaking group on the same day(P<0.05),while the contents of C5aR and IL-6 did not change significantly(P>0.05).The contents of C3a,C5aR,PGE2,PGF-2a,LZM and IL-6 in the same concentration were found to be increased first and then decreased with the extension of soaking time,with the maximal content in group D.The histological sections showed that the gill filaments of the fish were normal without lesions on day 0.On the 9th day of the experiment,there was no swelling at the end of gill filament in each immersion group due to the increase in blood cells,and there was no increase in inflammatory cells.In the control group,the gill filaments were red,swollen and festered.It can be seen that this traditional Chinese medicine formula can regulate the inflammatory factors and enhance non-specific immunity in koi carp.In general,the better effect was observed at immersion concentration of 250 mg·L^(-1).
作者
马娇娇
许斌
韦永春
曹鑫盛
张震
张其杰
陈成勋
MA Jiaojiao;XU Bin;WEI Yongchun;CAO Xinsheng;ZHANG Zhen;ZHANG Qijie;CHEN Chengxun(Tianjin Key Laboratory of Aqua-ecology and aquaculture,Tianjin Agricultural University,Tianjin 300380,China;Tianjin Longjin Aquaculture Limited Company,Tianjin 300382 China;Tianjin Zanlan Technology Limited Company,Tianjin 300380,China)
出处
《水产学杂志》
CAS
北大核心
2023年第2期21-27,共7页
Chinese Journal of Fisheries
基金
天津市淡水养殖产业技术体系创新团队建设项目(ITTFRS2021000)。
关键词
锦鲤疾病预防
青蒿槟榔碱混合制剂
非特异性免疫因子
鳃丝病变
disease prevention of koi carp Cyprinus carpio
Areca catechu and Artemisia carvifolia mixed preparation
nonspecific immune factor
branchial filament lesion
作者简介
马娇娇(1998-),女,汉族,硕士研究生,研究方向为水产动物饲料与营养.E-mail:2907264911@qq.com;通信作者:陈成勋(1967-),男,汉族,研究员,硕士生导师,研究方向为水产增养殖.E-mail:ccxnxy@163.com。
