摘要
目的:研究等温扩增技术发展态势,为我国开展相关研究提供数据参考。方法:通过文献计量方法、科学知识图谱等方法对等温扩增技术及新型冠状病毒(SARS-CoV-2)等温扩增检测相关的研究论文进行数据分析和知识挖掘,研究技术发展脉络、研究热点和发展趋势。结果:中国在等温扩增技术研究上具有较强的研究基础,SARS-CoV-2等温扩增检测技术研究处于全球领先水平。中国科学院等国内机构成为全球等温扩增技术的重要研究力量。研究较多的等温扩增技术主要为环介导等温扩增(LAMP)、滚环扩增(RCA)、重组酶聚合酶扩增(RPA)和依赖核酸序列的扩增(NASBA)。主要的研究对象为SARS-CoV-2、HIV、HPV、Salmonella和Malaria等病毒和细菌及癌症标记物。重点关注的技术工具为CRISPR/Cas基因编辑、生物传感器、微流控芯片等。结论:2000年以前,等温扩增技术主要集中于NASBA和链替代扩增(SDA)技术;2000年以后,LAMP、RCA等新技术出现,LAMP迅速成为主流技术。新型冠状病毒肺炎疫情发生以来,等温扩增技术广泛应用于SARS-CoV-2的快速性和便捷性检测。
Objective:To analyze the development of isothermal amplification technology,so as to provide data reference for relevant studies in China.Methods:Based on the bibliometrics analysis and scientific knowledge map,the research papers in the field of sothermal amplification technology and isothermal amplification technology of SARS-CoV-2 were statistically calculated,to discuss the technical development vein,research hotspots and development trend.Results:The results showed that China had a strong research foundation in isothermal amplification technology,and isothermal amplification detection technology of SARS-CoV-2 of Chinese research institutions such as the Chinese Academy of Sciences was at the leading level in the world.Loop-mediated isothermal amplification(LAMP),rolling circle amplification(RCA),recombinase polymerase amplification(RPA),and nucleic acid sequence-based amplification(NASBA)were the most studied isothermal amplification techniques.The main research objects were SARS-CoV-2,HIV,HPV,Salmonella,Malaria,or other viruses,bacteria,and cancer markers.The focus were on CRISPR/Cas gene editing,Biosensor,Microfluidic chips,etc.Conclusion:Before 2000,isothermal amplification techniques were mainly concentrated on NASBA and strand displacement amplification(SDA).After 2000,new techniques such as LAMP and RCA appeared,and LAMP quickly became the mainstream technology.Isothermal amplification had been widely used in the rapid and convenient detection of SARS-CoV-2 since the outbreak of COVID-19.
作者
张丽月
王姗姗
何祯硕
闫君
卢晅
刘万红
ZHANG Liyue;WANG Shanshan;HE Zhenshuo;YAN Jun;LU Xuan;LIU Wanhong(Hubei Province Key Laboratory of Allergy and Immunology,Taikang Medical School(School of Basic Medical Sciences),Wuhan University,Wuhan 430071,Hubei,China)
出处
《武汉大学学报（医学版）》
CAS
2023年第6期647-653,共7页
Medical Journal of Wuhan University
基金
国家自然科学基金资助项目(编号:52073022)。
关键词
等温扩增技术
知识图谱
研究热点
文献计量学
新型冠状病毒
Isothermal Amplification Technology
Knowledge Graph
Research Hotspot
Bibliometrics
SARS-CoV-2
作者简介
张丽月,女,1985-,生物医学工程硕士生,助理实验师,主要从事生物医学工程的研究,E-mail:pq002463@whu.edu.cn;通讯作者:卢晅,男,1976-,理学博士,讲师,主要从事分子病毒学的研究,E-mail:luxuan@whu.edu.cn;通讯作者:刘万红,男,1969-,理学博士,教授,主要从事病毒免疫与分子病毒学的研究,E-mail:liuwanhong@whu.edu.cn。
