摘要
目的研究鲜地黄水提与榨汁提取物UPLC特征图谱的差异性。方法以鲜地黄为原料,采用水提和榨汁两种工艺制备鲜地黄提取物;以乙腈-0.1%磷酸水溶液为流动相梯度洗脱,流速为0.3 mL/min,检测波长为203 nm,柱温为30℃,测定各批次鲜地黄水提与榨汁提取物的特征图谱,并结合多元统计分析、偏最小二乘法分析(OPLS-DA)、主成分分析(PCA)和聚类分析(CA)等化学模式识别方法比较二者的差异。结果鲜地黄提取物特征图谱共标定了10个共有峰,并对峰3(梓醇)、峰4(地黄苷D)、峰6(益母草苷)进行了指认;多元统计分析、OPLS-DA表明峰1、峰2、峰5、峰7、峰9、峰10的峰面积是导致鲜地黄水提和榨汁提取物质量差异的最主要因素;PCA和CA均能将鲜地黄水提和榨汁提取物单独聚为两类。结论建立的鲜地黄提取物UPLC特征图谱能够全面地反映鲜地黄水提和榨汁提取物之间化学成分的差异,可为鲜地黄现代制剂的制备工艺、质量控制以及临床使用提供参考。
Objective To study the difference of UPLC characteristic chromatogram between the fresh R.glutinosa extract powders prepared by decocting and juicing.Methods Using fresh R.glutinosa as raw material,the fresh R.glutinosa extract powders were prepared by two processes of decocting and juicing;Gradient elution with acetonitrile-0.1%phosphoric acid aqueous solution as mobile phase,flow rate was 0.3 mL/min,detection wavelength was 203 nm,column temperature was 30℃,determined the characteristic spectrum of each batch of fresh R.glutinosa extract powders.Combined with chemical pattern recognition methods such as multivariate statistical analysis,OPLS-DA,PCA and CA,the differences of fresh R.glutinosa extract powders prepared by decocting and juicing were compared.Results A total of 10 common peaks were calibrated in the characteristic chromatogram of the extract powders of fresh R.glutinosa,and peak 3(catalpoi),peak 4(rehmannioside),peak 6(leonuride)were identified;Multivariate statistical analysis and OPLS-DA showed that the area of peak 1,peak 2,peak 5,peak 7,peak 9 and peak 10 were the most important factors leading to the difference in quality between fresh R.glutinosa extract powders prepared by decocting and juicing.At the same time,PCA and CA could separate fresh R.glutinosa extract powders prepared by decocting and juicing into two categories.Conclusion The established UPLC characteristic chromatogram can comprehensively and truly reflect the chemical composition differences of the fresh R.glutinosa extract powders prepared by decocting and juicing,which can provide reference for the preparation technology,quality control and clinical use of modern prepartion of fresh R.glutinosa.
作者
鲁云
黄梦婷
徐杰
谢明晏
彭邦贵
程学仁
孙冬梅
LU Yun;HUANG Mengting;XU Jie;XIE Mingyan;PENG Banggui;CHENG Xueren;SUN Dongmei(Guangdong Yifang Pharmaceutical Co.,Ltd.,Guangdong Provincial Key Laboratory of Traditional Chinese Medicine Formula Granule,Foshan 528244,China)
出处
《现代中药研究与实践》
CAS
2023年第2期52-56,共5页
Research and Practice on Chinese Medicines
基金
广东省科技计划项目(2018B030323004)
广东特支计划科技创业领军人才项目(2017TY04R197)。
关键词
鲜地黄
提取物
特征图谱
化学模式识别
水提
榨汁
Fresh Rehmannia glutinosa
extract powder
characteristic chromatograms
chemical pattern recognition
decocting
juicing
作者简介
鲁云(1988-),女,硕士,副主任中药师,研究方向:中药配方颗粒质量与工艺研究。E-mail:lainyzz2015@163.com;通信作者:孙冬梅(1969-),女,博士研究生导师,主任中药师,研究方向:中药配方颗粒研究。E-mail:10268667@qq.com。
