摘要
目的探究微小核糖核酸21(miRNA-21)及微小核糖核酸181b(miRNA-181b)在精神分裂症患者外周血中的表达及意义。方法选取绍兴市第七人民医院2020年3月至2022年3月收治的精神分裂症患者100例为研究对象(研究组),另选取同期在该院体检的健康志愿者30例为对照组,检测比较两组血清miRNA-21、miRNA-181b表达水平。100例精神分裂症患者入科后均接受规范化治疗,采用阳性与阴性症状量表(PANSS)评估其精神症状,监测、收集、比较患者入科前及治疗后1周、4周、8周、12周血清miRNA-21、miRNA-181b表达水平及PANNS评分,绘制受试者工作特征(ROC)曲线分析血清miRNA-21、miRNA-181b表达水平对精神分裂症的诊断价值。结果研究组血清miRNA-21、miRNA-181b表达水平分别为(2.41±1.12)、(15.62±2.26),均显著高于对照组的(0.73±0.37)、(8.11±0.98)(t=8.07、17.67,均P<0.001);随治疗时间的延长,研究组血清miRNA-21、miRNA-181b表达水平及PANNS评分均逐渐降低(均P<0.001);血清miRNA-21、miRNA-181b检测诊断精神分裂症的ROC曲线下面积(AUC)分别为0.616、0.683,两者联合诊断精神分裂症的AUC为0.788,明显高于单独检测。结论miRNA-21、miRNA-181b在精神分裂症患者外周血中呈异常高表达,均可作为早期诊断精神分裂症的客观有效指标,两者联合准确性更高。
Objective To investigate the expression and significance of microRNA-21(miRNA-21)and microRNA-181b(miRNA-181b)in the peripheral blood of patients with schizophrenia.Methods A total of 100 patients with schizophrenia who received treatment in Shaoxing 7th People's Hospital from March 2020 to March 2022 were included in the study group.An additional 30 healthy controls who concurrently underwent physical examination were included in the control group.The expression of miRNA-21 and miRNA-181b in peripheral blood was compared between the two groups.The 100 patients with schizophrenia received standardized clinical treatment.Their mental symptoms were evaluated with the Positive and Negative Symptom Scale(PANSS).miRNA-21 and miRNA-181b expression and PANSS scores before and 1,4,8,and 12 weeks after treatment were collected and compared between the two groups.The receiver operating characteristic curve was plotted to analyze the value of miRNA-21 and miRNA-181b expression in the diagnosis of schizophrenia.Results Serum miRNA-21 and miRNA-181b expression in the study group were(2.41±1.12)and(15.62±2.26),respectively,which were significantly higher than(0.73±0.37)and(8.11±0.98)in the control group(t=8.07,17.67,both P<0.05).With the prolongation of treatment time,serum miRNA-21 and miRNA-181b expression and PANSS score in the study group gradually decreased(all P<0.001).The area under the receiver operating characteristic curve plotted for evaluating the value of miRNA-21 and miRNA-181b expression in the diagnosis of schizophrenia was 0.616 and 0.683,respectively.The area under the receiver operating characteristic curve plotted for evaluating the value of miRNA-21 combined with miRNA-181b expression in the diagnosis of schizophrenia was 0.788,which was markedly higher than that for the detection of miRNA-21 or miRNA-181b expression alone.Conclusion miRNA-21 and miRNA-181b are abnormally highly expressed in the peripheral blood of patients with schizophrenia.Both of them can be used as objective and effective indicators for early diagnosis of schizophrenia.Combined detection of miRNA-21 and miRNA-181b provides higher accuracy in the diagnosis of schizophrenia than the detection of miRNA-21 or miRNA-181b alone.
作者
王鹭
曹峰
陈华
Wang Lu;Cao Feng;Chen Hua(Department of Psychiatry,Shaoxing 7th People's Hospital,Shaoxing 312000,Zhejiang Province,China)
出处
《中国基层医药》
CAS
2023年第4期596-600,共5页
Chinese Journal of Primary Medicine and Pharmacy
基金
浙江省绍兴市医卫类科技计划(2020A13051)。
作者简介
通信作者:王鹭,Email:568350420@qq.com。
