摘要
作为我国辽西北主要的造林树种,樟子松在当地发挥着重要的生态环境效益。但近年来,干旱频发,樟子松出现大面积的衰退甚至死亡现象。为研究樟子松(Pinus sylvestris var.mongolica)对不同程度干旱胁迫的响应以及干旱对枯梢病发生的影响,本研究采用盆栽控水试验模拟3种水分条件(CK:80%FWC;中度干旱:40%FWC;重度干旱:20%FWC)分析了不同程度干旱胁迫下樟子松幼苗主根、侧根、茎和针叶的部分生理生化指标以及樟子松枯梢病的发病情况。结果表明:随着干旱胁迫程度的加剧,樟子松幼苗的针叶相对电导率逐渐下降;超氧阴离子(O^(ˉ)_(2))、过氧化氢(H2O2)以及丙二醛(MDA)的含量随着干旱胁迫的加剧,不同器官呈现不同程度的上升趋势;针叶氮蓝四唑(NBT)和伊文思蓝(Evans blue)染色也随之加深。樟子松各器官的超氧化物歧化酶(SOD)活性、过氧化物酶(POD)、过氧化氢酶(CAT)活性则随着干旱胁迫程度的加剧呈现先上升后下降的趋势。同时,在干旱胁迫下,樟子松枯梢病的发病率及病情指数均显著上升,且上升幅度与干旱胁迫程度呈正比。初步说明,干旱胁迫导致樟子松细胞膜受损,且随着干旱胁迫程度的加剧,膜脂过氧化程度加深。严重干旱胁迫下,植株细胞的抗氧化酶活性显著降低。此外,干旱胁迫显著降低了樟子松对枯梢病的抗性,严重抑制樟子松的生长发育。
As the main afforestation tree species in northwestern Liaoning,Pinus sylvestris var.mongolica plays an important role in local ecological and environmental benefits.However,in recent years,drought has occurred frequently,and Pinus sylvestris var.mongolicahas experienced widespread decline or even death.In this study,pot water control experiment was used to simulate three water conditions(CK:80% FWC;moderate drought:40% FWC;heavy drought:20% FWC) to analyze some physiological and biochemical indexes of primary root,lateral root,stem and needle of Pinus sylvestris var.mongolica seedlings under different degrees of drought stress and the incidence of Pinus sylvestris var.mongolica dieback.The results showed that the relative conductivity of needles decreased gradually with the intensification of drought stress.With the intensification of drought stress,the contents of superoxide anion(O^(ˉ)_(2)),hydrogen peroxide(H_(2)O_(2)) and malondialdehyde(MDA) in different organs showed different degrees of upward trend.The staining of NBT and Evans blue also deepened.The activities of SOD,POD and CAT in each organ of Pinus sylvestris var.mongolica increased first and then decreased with the deepening of drought stress.At the same time,under drought stress,the incidence rate and disease index of Pinus sylvestris var.mongolica dieback disease increased significantly,and the increase was proportional to the degree of drought stress.The preliminary results showed that drought stress caused damage to the cell membrane of Pinus sylvestris var.mongolica,and with the intensification of drought stress,the degree of membrane lipid peroxidation increased.Under heavy drought stress,the antioxidant enzyme activity of plant cells decreased significantly.In addition,drought stress significantly reduced the resistance of Pinus sylvestris var.mongolica to shoot blight,and seriously inhibited the growth and development of Pinus sylvestris var.mongolica.
作者
尹大川
孟凡君
张天泽
YIN Da-chuan;MENG Fan-jun;ZHANG Tian-ze(College of Forestry,Shenyang Agricultural University,Shenyang 110161,China)
出处
《沈阳农业大学学报》
CAS
CSCD
北大核心
2023年第1期36-45,共10页
Journal of Shenyang Agricultural University
基金
国家自然科学基金项目(31800542)
辽宁省教育厅面上项目(2021)。
关键词
樟子松
相对电导率
活性氧
抗氧化酶
松枯梢病
Pinus sylvestris var.mongolica
relative conductivity
ractive oxygen species
antioxidant enzyme
shoot blight
作者简介
第一作者:尹大川(1987-),男,博士,副教授,从事森林病理学研究,E-mail:yindachuan@syau.edu.cn。
