摘要
目的采用气相色谱-质谱联用技术(GC-MS)分析超临界萃取法提取的艾纳香叶中的挥发性成分。方法采用CO_(2)超临界萃取技术提取艾纳香叶中的挥发性成分,并采用GC-MS技术分析鉴定其化学成分,通过峰面积归一化法计算各成分的相对含量。结果从艾纳香叶CO_(2)超临界提取物中共分离出119个化学成分,鉴定出78种成分,占艾纳香叶CO_(2)超临界提取物中挥发性成分总量的87.02%,主要包括5种萜类衍生物(31.90%)、44种脂肪族化合物(18.58%)、10种芳香族化合物(18.50%)、12种含氮化合物(14.54%)及部分其它成分。结论CO_(2)超临界萃取是一种新型的中药提取技术,日益广泛地应用于挥发性成分的提取工艺中,本研究采用GC-MS技术对艾纳香叶CO_(2)超临界提取物中的化学成分进行了分析,可以为今后艾纳香叶化学成分的提取及应用提供参考。
Objective Gas chromatography-mass spectrometry(GC-MS)was used to analyze the volatile components in the leaves of Blumea balsamifera extracted by supercritical extraction.Methods The volatile components were extracted by CO_(2)supercritical extraction technology and identified by GC-MS.The relative contents of each component were calculated by peak area normalization method.Results A total of 119 chemical components were isolated and 78 components were identified from CO_(2)supercritical extraction of B.balsamifera leaves,accounting for 87.02%of the total volatile components.There were 5 terpenoid derivatives(31.90%),44 aliphatic compounds(18.58%),10 aromatic compounds(18.50%),12 nitrogenous compounds(14.54%)and some other compounds.Conclusion CO_(2)Supercritical extraction is a new extraction technology of Traditional Chinese medicine,which is increasingly widely used in the extraction process of volatile components.In this study,GC-MS technology was used to analyze the chemical components in CO_(2)supercritical extraction from the leaves of B.balsamifera,which can provide reference for the extraction and application of chemical components in the leaves of B.balsamifera in the future.
作者
王国盼
杨达
陆安静
秦琳
张倩茹
王建梅
杨再文
何莉
谭道鹏
Wang Guopan;Yang Da;Lu Anjing;Qin Lin;Zhang Qianru;Wang Jianmei;Yang Zaiwen;He Li;Tan Daopeng(Guizhou Engineering Center of Industrial Key-Technology for Dendrobium nobile,Zunyi Medical University,Zunyi Guizhou 563000,China;Guizhou Huiteng Technology Co.,Ltd.,Zunyi Guizhou 563000,China;Guizhou Miao Medicine Biotechnology Co.,Ltd.,Tongren Guizhou 554400,China)
出处
《遵义医科大学学报》
2023年第4期415-423,共9页
Journal of Zunyi Medical University
基金
贵州省研究生科研基金项目,黔教合YJSKYJJ[2021]189
贵州省科技支撑项目,黔科合支撑[2021]一般476。
关键词
艾纳香
CO
2超临界萃取
气相色谱-质谱联用技术
挥发性成分
Blumea balsamifera
CO_(2)supercritical extraction
gas chromatography-mass spectrometry
volatile components
作者简介
通信作者:谭道鹏,男,博士,副教授,研究方向:中药活性成分、质量标准及其创新药物研究,E-mail:tdp2003@163.com。
