摘要
果形和糖含量分别是与瓜类蔬菜作物外观、营养及风味密切相关的重要品质性状。为探究黄瓜倍性差异导致果形及糖含量变化的分子机制,以果形和糖含量差异显著的黄瓜二倍体(长果/低糖)和四倍体(短果/高糖)为试材,分别取开花当天和花后9 d的果实进行转录组分析。结果表明,在二、四倍体花后9 d果实中共鉴定到1874个差异表达基因(DEGs),其中上调基因818个和下调基因1056个;GO分析显示,DEGs显著富集在“DNA复制”(GO:0006260)、“DNA代谢过程”(GO:0006259)、“蛋白质复合体”(GO:0032993)、“染色体”(GO:0005694)、“蛋白质二聚活性”(GO:0046983)等GO条目上;KEGG分析表明,DEGs富集在“植物激素信号转导”(csv04075)、“淀粉与蔗糖代谢”(csv00500)等代谢通路上;参与“植物激素信号转导”相关基因AUX1、DELLA、B-ARR、TCH4上调表达,GH3、GID1、PP2C、CYCD3下调表达,以及参与“淀粉与蔗糖代谢”相关基因SPS、SUSY上调表达,可能与果形指数减小和果实糖含量增加有关。荧光定量PCR(RT-qPCR)分析显示,除ARF4外,所选定DEGs的表达模式与RNA-Seq中表达趋势相一致,其中SUSY在二、四倍体黄瓜果实中差异表达极显著,推测该基因在调控黄瓜四倍体果形及糖含量形成过程中起到重要作用。
Fruit shape and sugar content are important quality traits closely related to the appearance,nutrition and flavor of melon vegetables,respectively.To investigate the molecular mechanism of fruit shape and sugar content in cucumber caused by ploidy differences,the diploid(long fruit/low sugar)and autotetraploid(short fruit/high sugar)cucumber with significant differences of shape and sugar content were selected as the study materials,and RNA-seq analysis on the fruits at 0 and 9 d after flowering were performed.The results showed that a total of 1 874 differentially expressed genes(DEGs)were identified from diploid and autotetraploid at 9 d after flowering,including 818up-regulated genes and 1 056 down-regulated genes.GO analysis demonstrated that the DEGs were significantly enriched in DNA replication(GO:0006260),DNA metabolic process(GO:0006259),protein-DNA complex(GO:0032993),chromosome(GO:0005694),and protein dimerization activity(GO:0046983).KEGG analysis revealed that the DEGs were enriched in “plant hormone signal transduction”(csv04075),“starch and sucrose metabolism”(csv00500)and other metabolic pathways.The up-regulated expressions of AUX1,DELLA,B-ARR and TCH4and down-regulated expression of GH3,GID1,PP2C and CYCD3 related to “plant hormone signal transduction”,as well as the up-regulated expression of SPS and SUSY related to “starch and sucrose metabolism”,may correlate with fruit shape index decreasing shortening and sugar content increasing in the cucumber.Quantitative reverse transcription-PCR(RT-qPCR)analysis showed that the expression pattern of selected DEGs was consistent with the expression trend of RNA-Seq except for ARF4,among which SUSY was highly differentially expressed between diploid and autotetraploid cucumber fruits,suggesting that SUSY plays a critical role in regulating fruit shape and sugar content in cucumber autotetraploid.
作者
谢洋
邢雨蒙
周国彦
刘美妍
银珊珊
闫立英
XIE Yang;XING Yu-meng;ZHOU Guo-yan;LIU Mei-yan;YIN Shan-shan;YAN Li-ying(College of Horticulture Science and Technology,Hebei Normal University of Science and Technology,Qinhuangdao 066004;Hebei Key Laboratory of Horticultural Germplasm Excavation and Innovative Utilization,Qinhuangdao 066004)
出处
《生物技术通报》
CAS
CSCD
北大核心
2023年第3期152-162,共11页
Biotechnology Bulletin
基金
河北省自然科学基金项目(C2022407013)
河北省高校园艺作物育种应用技术研发中心(YF201404)。
关键词
黄瓜
同源四倍体
转录组
差异表达基因
cucumber
autotetraploid
transcriptome
differentially expressed genes
作者简介
通讯作者:谢洋 ,女,博士,讲师,研究方向:蔬菜栽培生理及分子生物学,E-mail:xieyangly123@163.com。
