摘要
为获得花椒根腐病菌腐皮镰孢菌Fusarium solani的优良拮抗菌株,本研究以健康花椒侧根为拮抗菌来源,采用组织分离法获得花椒根部内生真菌,通过平板对峙法筛选出1株对病原菌具备明显拮抗作用的真菌HB21-1,平板抑制率为61.00%,其无菌发酵滤液抑制率为41.24%,可致腐皮镰孢菌菌丝出现皱缩膨胀、孢子畸形现象,经形态学和分子生物学鉴定为草酸青霉Penicilium oxalicum。盆栽试验结果表明HB21-1对花椒根腐病的防效为56.59%,经HB21-1处理的花椒根部POD、SOD酶活性和土壤蔗糖酶、酸性磷酸酶活性随时间均呈升高趋势,且始终高于空白对照。本研究表明草酸青霉HB21-1对花椒根腐病病原菌具有显著拮抗作用,能降低发病率,在生物防治方面有潜在应用价值。
In order to obtain good antagonistic strains against Fusarium solani,the endophytic fungus of Zanthoxylum bungeanum root was screened by tissue isolation method.The strain HB21-1 with inhibition rate of61.00% against F.solani was obtained by plate confrontation method.The inhibition rate of its sterile fermentation filtrate against F.solani was 41.24%.The strain of HB21-1 induced constriction,enlargement in mycelia morphology of F.solani and caused spore deformity.Based on the morphological characteristic and ITS molecular sequences,The strain of HB21-1 was identified as Penicillium oxalicum.The effect of HB21-1 on root rot of Zanthoxylum bungeanum was 56.59% in the pot experiment.The POD and SOD enzyme activities,soil sucrase and acid phosphatase activities of Zanthoxylum bungeanum roots treated with HB21-1 were increased compared with the control.This study shows that P.oxalicum HB21-1 had a significant antagonistic effect on F.solani and reduced the incidence of the root rot of Zanthoxylum bungeanum.
作者
李梦玮
陈晓霞
补欢欢
廖礼彬
李婧怡
张楠楠
石福孙
LI Mengwei;CHEN Xiaoxia;BU Huanhuan;LIAO Libin;LI Jingyi;ZHANG Nannan;SHI Fusun(Chengdu Institute of Biology,Chinese Academy of Sciences,Chengdu 610041,China;University of Chinese Academy of Sciences,Beijing 100049,China;Maoxian Mountain Ecosystem Research Station,Chinese Academy of Sciences,Maoxian 623200,China)
出处
《中国生物防治学报》
CSCD
北大核心
2023年第1期176-183,共8页
Chinese Journal of Biological Control
基金
四川省科技计划项目(2021YFN0116)。
关键词
花椒
根腐病
草酸青霉
生物防治
防御酶活性
土壤酶活性
Zanthoxylum bungeanum
root rot
Penicillium oxalicum
biological control
defense enzyme activity
soil enzyme activity
作者简介
李梦玮,硕士研究生,E-mail:limw@cib.ac.cn;通信作者:张楠楠,助理研究员,E-mail:zhangnn@cib.ac.cn;通信作者:石福孙,研究员,E-mail:shifs@cib.ac.cn。
