摘要
为探索促性腺激素释放激素(gonadotropin-releasing hormone,GnRH)基因亚型GnRH-1基因与鹌鹑蛋品质的相关性,利用PCR扩增和DNA测序技术,检测GnRH-1基因在中国黄羽鹌鹑、北京白羽鹌鹑、朝鲜鹌鹑中的单核苷酸多态性(SNP)突变位点,并分析GnRH-1基因的多态性与鹌鹑蛋品质的关联性,以期获得鹌鹑蛋品质相关的分子标记,为蛋用鹌鹑新品系的培育提供参考。结果表明,GnRH-1基因在中国黄羽鹌鹑、北京白羽鹌鹑、朝鲜鹌鹑中检测到14个SNP突变位点,分别为C71T、C108T、C168T、C178T、A184G、C206T、A209C、C215T、A252G、A279T、C281T、C293G、C339T、C458T,除A209C和C281T在中国黄羽鹌鹑和北京白羽鹌鹑中只检测到2种基因型外,其他12个SNP位点在3个鹌鹑品种中均检测到3种基因型。关联分析表明,GnRH-1基因的C71T、C215T、A209C、A279G位点与鹌鹑的蛋黄高度和蛋黄指数有显著(P<0.05)相关性,A209C位点与鹌鹑的蛋黄宽度有显著(P<0.05)相关性,A279G位点与鹌鹑的蛋壳厚度有显著(P<0.05)相关性。综上所述,GnRH-1基因可以作为影响鹌鹑蛋品质的分子标记或候选基因。
To explore the correlation between GnRH-1 gene of gonadotropin releasing hormone(GnRH)gene subtype and quail egg quality,the single nucleotide polymorphism(SNP)mutation site of GnRH-1 gene in Chinese yellow quails,Beijing white quails and Korean quails was detected by PCR amplification and DNA sequencing,the polymorphism of GnRH-1 gene and its association with quality of quail eggs were analyzed in order to obtain the molecular markers related to the quality of quail eggs.The results showed that 14 SNP mutations of GnRH-1 gene were detected in Chinese yellow quails,Beijing white quails and Korean quails,which were C71T,C108T,C168T,C178T,A184G,C206T,A209C,C215T,A252G,A279T,C281T,C293G,C339T,C458T.Only two genotypes of A209C and C281T were detected in Chinese yellow quail and Beijing white quail,while other 12 SNP loci detected three genotypes in three quail varieties.Correlation analysis showed that there were significant(P<0.05)correlations between C71T,C215T,A209C and A279G loci of GnRH-1 gene and yolk height and yolk index,and between the A209C locus and the yolk width.There was a significant(P<0.05)correlation between A279G locus and eggshell thickness of quail.In conclusion,GnRH-1 gene could be used as a molecular marker or candidate gene for quail egg quality.
作者
王龙威
白俊艳
贾小平
雷莹
陈梦柯
樊红灯
卢小宁
何豫涵
曾凡林
张容恺
WANG Longwei;BAI Junyan;JIA Xiaoping;LEI Ying;CHEN Mengke;FAN Hongdeng;LU Xiaoning;HE Yuhan;ZENG Fanlin;ZHANG Rongkai(College of Animal Science and Technology,Henan University of Science and Technology,Luoyang 471023,Henan,China;Luoyang Key Laboratory of Animal Genetics and Breeding,Luoyang 471023,Henan,China;College of Agriculture/Tree Peony,Henan University of Science and Technology,Luoyang 471023,Henan,China)
出处
《浙江农业学报》
CSCD
北大核心
2023年第3期565-574,共10页
Acta Agriculturae Zhejiangensis
作者简介
王龙威(1998-),男,河南周口人,硕士研究生,主要从事动物分子遗传育种研究。E-mail:1575880518@qq.com;通信作者:白俊艳,E-mail:junyanbai@163.com。
