摘要
目的评估低强度电刺激(LIES)对神经损伤性勃起功能障碍(ED)大鼠勃起功能(EF)影响。方法雄性SD大鼠随机分为3组:Sham组、双侧海绵体神经损伤(BCNI)组和LIES+BCNI组,每组10只大鼠。LIES治疗7 d后,通过对比大鼠最大海绵体内压/平均动脉压(ICP_(max)/MAP)评估阴茎EF变化;通过ELISA试剂盒测量海绵体内活性氧(ROS)蛋白含量;通过Western blot方法检查海绵体中转化生长因子-β1(TGF-β1)、白细胞介素-6(IL-6)和C反应蛋白(CRP)蛋白表达;通过免疫荧光染色评估海绵体中α-平滑肌肌动蛋白(α-SMA)蛋白表达。结果与Sham组相比,BCNI组大鼠ICP_(max)/MAP降低(P<0.05);与BCNI组比,BCNI+LIES组大鼠ICP_(max)/MAP升高(P<0.05)。与Sham组相比,BCNI组大鼠海绵体ROS含量增高(P<0.05);与BCNI组比,BCNI+LIES组大鼠海绵体ROS含量降低(P<0.05)。与Sham组相比,BCNI组大鼠海绵体TGF-β1、IL-6和CRP蛋白表达增加(P<0.05);与BCNI组比,BCNI+LIES组大鼠海绵体TGF-β1、IL-6和CRP蛋白表达降低(P<0.05)。与Sham组相比,BCNI组海绵体内α-SMA蛋白表达降低(P<0.05);与BCNI组比,BCNI+LIES组大鼠海绵体α-SMA蛋白表达增加(P<0.05)。结论LIES治疗不仅改善BCNI后大鼠EF,而且对海绵体组织具有保护作用,包括防止氧化应激、炎症和平滑肌细胞丢失。
【Objective】To evaluate the effect of low intensity electrical stimulation(LIES)on erectile function(EF)in rats with nerve injury induced erectile dysfunction(ED).【Methods】Male SD rats were randomly divided into 3 groups:Sham group,bilateral cavernous nerve injury(BCNI)group and LIES+BCNI group,with 10 rats in each group.After 7 days of treatment with LIES,the changes of penile EF were evaluated by comparing the maximum intracavernous pressure/mean arterial pressure(ICP_(max)/MAP)of rats.The content of reactive oxygen species(ROS)protein in cavernous body was measured by ELISA kit.The protein expression of transforming growth factor-β1(TGF-β1),interleukin-6(IL-6)and C-reactive protein(CRP)in cavernous body was examined by Western blot.Immunofluorescence staining was used to evaluate theα-smooth muscle actin(α-SMA)protein expression.【Results】Compared with Sham group,ICP_(max)/MAP of rats in BCNI group decreased(P<0.05);compared with BCNI group,ICP_(max)/MAP in BCNI+LIES group increased(P<0.05).Compared with Sham group,the content of ROS in cavernous body of rats in BCNI group increased(P<0.05);compared with BCNI group,the content of ROS in cavernous body of rats in BCNI+LIES group decreased(P<0.05).Compared with Sham group,the expression of TGF-β1,IL-6 and CRP protein in cavernous body of rats in BCNI group increased(all P<0.05);compared with BCNI group,the expression of TGF-β1,IL-6 and CRP protein in cavernous body of rats in BCNI+LIES group decreased(all P<0.05).Compared with Sham group,the expression ofα-SMA protein in BCNI group was decreased(P<0.05);compared with BCNI group,theα-SMA protein expression in cavernous body of BCNI+LIES group was increased(P<0.05).【Conclusion】LIES treatment not only improved EF in rats after BCNI,but also had protective effects on cavernous body,including prevention of oxidative stress,inflammation,and smooth muscle cell loss.
作者
李鸿斌
冯东
侯琳
邢建东
张延兵
LI Hongbin;FENG Dong;HOU Lin;XING Jiandong;ZHANG Yanbing(Department of Urology,the Fifth People's Hospital of Datong,Datong,Shanxi 037006,China;Department of Endocrinology,the Fifth People's Hospital of Datong,Datong,Shanxi 037006,China)
出处
《中国医学工程》
2023年第3期14-18,共5页
China Medical Engineering
基金
山西省重点研发计划项目(201903D321157)。
关键词
电刺激
勃起功能障碍
海绵体
大鼠
electrical stimulation
erectile dysfunction
cavernous body
rats
