摘要
阿特拉津是一种三嗪类除草剂,具有内分泌干扰效应,其在厌氧环境中的半衰期较好氧环境更长,会对生态环境和人体健康构成威胁.为此,本研究筛选出厌氧条件下普遍存在的硫酸盐还原菌菌株希瓦氏菌(Shewanella sp.JN01),在明确该菌株的最适生长条件和阿特拉津浓度的基础上,开展了不同温度(250、400和600℃)所制备的生物炭对Shewanella sp.JN01厌氧降解水体系中阿特拉津影响的研究.结果显示,生物炭、Shewanella sp.JN01及两者联合对阿特拉津的最大去除率分别为41.64%、70.52%和88.25%.当菌株/生物炭为2∶1(体积比)时,由玉米芯为原材料在250℃下制备的生物炭因具有较多的脂肪碳而更有效地促进了菌株的生长.通过qPCR检测Shewanella sp.JN01中的阿特拉津降解基因发现其含有atzA基因,但生物炭对atzA基因的表达水平无显著影响.因此,生物炭主要通过促进Shewanella sp.JN01的生长而实现对阿特拉津的有效降解.本研究结果可为利用微生物强化去除厌氧体系中的阿特拉津提供理论依据和数据支撑.
Atrazine is a triazine herbicide with endocrine disrupting effect.Its half-life is longer in anaerobic condition than that in aerobic condition,which poses a threat to the ecological environment and human health.Therefore,the sulfate reducing bacteria strain Shewanella sp.JN01,which is ubiquitous under anaerobic condition,was screened out for this study.Based on the optimum growth conditions of the strain and atrazine concentration,the effects of biochars prepared at different temperatures(250,400 and 600℃)on anaerobic biodegradation of atrazine by Shewanella sp.JN01 in aqueous system were studied.The results showed that the maximum removal efficiency of atrazine by biochar,Shewanella sp.JN01 and the combination of both were 41.64%,70.52%and 88.25%,respectively.When the strain/biochar ratio was 2∶1,the biochar prepared from corn cobs at 250℃promoted the growth of the strain more effectively because it had more aliphatic carbon.The atrazine biodegradation gene atzA in Shewanella sp.JN01 was detected by qPCR.However,the biochar had no significant effect on the expression level of atzA gene.Accordingly,the biochar can effectively biodegrade atrazine mainly by promoting the growth of Shewanella sp.JN01.The results of this study will provide a theoretical basis and data support for the enhanced removal of atrazine from anaerobic systems using microorganisms.
作者
吉莉
赵雅娜
李娜
张桂香
李渊
李雯雯
JI Li;ZHAO Yana;LI Na;ZHANG Guixiang;LI Yuan;LI Wenwen(School of Environment and Resources,Taiyuan University of Science and Technology,Taiyuan 030024)
出处
《环境科学学报》
CAS
CSCD
北大核心
2023年第3期104-115,共12页
Acta Scientiae Circumstantiae
基金
国家自然科学基金面上项目(No.42177057)
山西省基金面上项目(No.20210302123204)。
作者简介
吉莉(1982-),女,E-mail:jili@tyust.edu.cn;责任作者:张桂香,E-mail:zhanggx@tyust.edu.cn。
