摘要
目的观察针刺不同穴位及不同针刺条件下的穴位局部理化特征。方法实验1将24只SD大鼠随机分为4组,每组6只,观察针刺不同经穴及非经非穴的穴位局部理化特征;实验2选取6只SD大鼠,观察肾俞穴针刺不同时长(5 min、10 min、15 min、20 min、30 min)的局部血流灌注量;实验3选取6只SD大鼠,观察肾俞穴在起针后(0 min、5 min、10 min、15 min、20 min、30 min)的局部血流灌注量。结果针刺不同穴位的局部理化特征为,针刺可增加肾俞、百会、足三里穴位局部血流灌注量及穴位局部乳酸(lactic acid,Lac)、丙氨酸(alanine,Ala)、磷酸肌酸(phosphocreatine,PCr)、葡萄糖(glucose,Glu)能量代谢水平。针刺肾俞穴不同时长的穴位局部理化特征为,与针刺前比较,肾俞穴在留针后5 min、10 min其局部血流灌注量明显上升(P<0.01),之后平缓下降但仍较高(P<0.05);与留针5 min、10 min、15 min比较,留针30 min穴位局部血流灌注量降低(P<0.05)。针刺肾俞穴后效应的穴位局部理化特征为,与针刺前比较,肾俞穴在起针后6个时间点血流灌注量均显著升高(P<0.05);且与起针后5 min比较,起针15 min、20 min明显降低(P<0.05,P<0.01)。结论针刺不同穴位其局部血流灌注量与局部组织代谢改变不同,提示穴位的局部理化特征具有位置差异的特异性。针刺时长影响穴位局部血流灌注量,但是针刺时长10 min后穴位局部变化趋于平稳,同时,针刺肾俞穴存在后效应至少可持续30 min,为临床针刺效应提供参考依据。
Objective To observe the local physical and chemical characteristics of different acupoints under different needling conditions.Method In experiment 1,24 Sprague Dawley(SD)rats were randomized into 4 groups,with 6 rats in each group,to observe the topical physical and chemical characteristics of different acupoints and non-meridian non-acupoint spots after acupuncture.In experiment 2,6 SD rats were selected to measure the topical blood perfusion amount at Shenshu(BL23)at different time points after acupuncture(5 min,10 min,15 min,20 min,and 30 min).In experiment 3,6 SD rats were selected to observe the topical blood perfusion amount at Shenshu after needle removal(0 min,5 min,10 min,15 min,20 min,and 30 min).Result Study of the local physical and chemical characteristics of different acupoints after acupuncture showed that acupuncture increased local blood perfusion at Shenshu(BL23),Baihui(GV20),and Zusanli(ST36)and local energy metabolism levels of lactic acid(Lac),alanine(Ala),phosphocreatine(PCr),and glucose(Glu).Study of the local physical and chemical features after different time lengths of acupuncture at Shenshu revealed that the topical blood perfusion amount increased notably at 5 min and 10 min during needle remaining at Shenshu compared with that before acupuncture(P<0.01)and then remained at a relatively high level despite a steady downward tendency(P<0.05);the topical blood perfusion amount decreased at 30 min during needle remaining compared with that at 5 min,10 min,and 15 min(P<0.05).Study of the topical physical and chemical characteristics at Shenshu after needle removal showed that the blood perfusion amount at Shenshu increased significantly at all 6 time points after needle removal compared with that before acupuncture(P<0.05);the blood perfusion amount dropped substantially at 15 min and 20 min after needle removal compared with that at 5 min after needle removal(P<0.05,P<0.01).Conclusion The topical blood perfusion amount and tissue metabolism vary with acupuncture at different acupoints,suggesting a location-related acupoint specificity in topical physical and chemical characteristics.The time length of acupuncture affects topical blood perfusion amount at acupoints,though it tends to go steady after 10 min of needle remaining;further,the post-acupuncture effects at Shenshu can last for over 30 min.The above evidence supports clinical acupuncture effects.
作者
余燕
杨敏光
刘雨露
丁妍怡
许雯珊
江萍利
林华伟
柳维林
陈立典
YU Yan;YANG Minguang;LIU Yulu;DING Yanyi;XU Wenshan;JIANG Pingli;LIN Huawei;LIU Weilin;CHEN Lidian(College of Rehabilitation Medicine,Fujian University of Traditional Chinese Medicine,Fuzhou 350122,China;Rehabilitation Industry Institute of Fujian University of Traditional Chinese Medicine,Fuzhou 350122,China)
出处
《上海针灸杂志》
CSCD
2023年第3期306-311,共6页
Shanghai Journal of Acupuncture and Moxibustion
基金
国家自然科学基金项目(81873355)。
关键词
针刺疗法
穴位
激光斑点对比成像
血流灌注量
大鼠
Acupuncture therapy
Points
Laser speckle contrast imaging
Blood perfusion amount
Rats
作者简介
余燕(1998-),女,2019级硕士生,Email:yy2015611829@163.com;通信作者:柳维林(1985-),男,教授,博士,Email:liuweilin12@fjtcm.edu.cn。
