摘要
基于高效液相色谱(HPLC)法和网络药理学方法研究蒲公英中抗氧化活性成分及作用机制.采用石油醚、乙酸乙酯、二氯甲烷和正丁醇等有机试剂分别提取中草药蒲公英的有效成分,研究其抗氧化效果,同时采用网络药理学多个在线数据库获取蒲公英抗氧化共有靶点,构建PPI网络,并进行GO富集分析和KEGG信号通路富集分析.结果表明:蒲公英各相提取物对羟自由基(·OH)、超氧阴离子自由基(O^(2-)·)和1,1-二苯基-2-三硝基苯肼自由基(DPPH·)均有一定的清除能力,蒲公英乙酸乙酯相提取物和蒲公英二氯甲烷相提取物的清除效果较好,主要的抗氧化活性成分为异鼠李素和齐墩果酸;线上数据库筛选出蒲公英抗氧化靶点有137个,共有靶点主要集中在膜筏上,通过细胞对无机物、氮化物和营养水平的反应起抗氧化作用;共有靶点与癌症信号通路、NF-κappa B信号通路和PPAR信号通路等密切相关.
We studied the antioxidant active components and mechanism of dandelion based on high performance liquid chromatograph(HPLC)method and network pharmacology method.The effective components of Chinese herbal medicine dandelion were extracted by organic reagents,such as petroleum ether,ethyl acetate,dichloromethane and n-butanol,and their antioxidant effects were studied.Multiple online databases of network pharmacology were used to obtain common targets of dandelion antioxidant construct PPI network,and conduct GO enrichment analysis and KEGG signal pathway enrichment analysis.The results show that the extracts of each phase of dandelion have a certain scavenging ability to hydroxy radical(·OH),superoxide anion radical(O^(2-)·)and 1,1-dipheny l-2-picrylhydrazyl radical(DPPH·),the ethyl acetate phase extract of dandelion and the dichloromethane phase extract of dandelion have better scavenging effects,and the main antioxidant active components are isorhamnetin and oleanolic acid.There are 137 dandelion antioxidant targets screened from the online database.The common targets are mainly concentrated on membrane rafts,which are resistant to the response of cells to inorganic substances,nitrogen compounds,and nutrient levels oxidation.The common targets are closely related to cancer signal pathway,NF-kappa B signal pathway,and PPAR signal pathway.
作者
潘明月
李涛
陈婉钰
张晓颖
龙生
吴禹岐
于睿
张蕾
PAN Mingyue;LI Tao;CHEN Wanyu;ZHANG Xiaoying;LONG Sheng;WU Yuqi;YU Rui;ZHANG Lei(Department of Pharmaceutical Engineering,School of Life Science and Technology,Mudanjiang Normal University,Mudanjiang 157011,Heilongjiang Province,China)
出处
《吉林大学学报（理学版）》
CAS
北大核心
2023年第2期437-442,共6页
Journal of Jilin University:Science Edition
基金
黑龙江省基本科研业务费(批准号:1452PT007,1354MSYQN024,1354MSYQN025)
黑龙江省博士后基金(批准号:LBH-19190)
黑龙江省大学生创业实践项目(批准号:kjcx2021-082mdjnu)
黑龙江省大学生创新项目(批准号:S202210233032,MSYSYL2022019).
关键词
高效液相色谱
网络药理学
蒲公英
抗氧化
high performance liquid chromatography(HPLC)
network pharmacology
dandelion
antioxidant
作者简介
第一作者:潘明月(2000-),女,汉族,硕士研究生,从事生化药学的研究,E-mail:1765134763@qq.com;通信作者:张蕾(1984-),女,汉族,博士,副教授,从事微生物与生物化学的研究,E-mail:swxzlz@126.com.
