摘要
为研究粟酒裂殖酵母sgf73基因缺失后的关键基因和关键代谢通路,采用RNA-Seq测序技术对野生型酵母菌株及sgf73基因缺陷型菌株进行测序及生物信息学分析,并进行GO和KEGG功能富集分析.结果表明:sgf73基因缺陷型菌株中高表达基因有1834个,极高表达基因有6个,且有1714个差异表达基因,包括934个上调基因,780个下调基因;sgf73基因敲除导致细胞代谢和跨膜转运过程异常变化;MAPK信号通路中参与周期调控cki1,cki2和cdc25基因的下调导致sgf73Δ菌株有丝分裂时间延长;调控细胞骨架rgf2,rho1和stt4基因的下调导致sgf73Δ菌株肌动蛋白环收缩异常.
In order to study the key genes and key metabolic pathways after the sgf 73 gene was knocked out in Schizosaccharomyces pombe,the wild-type yeast strains and sgf73Δgene-deficient strains were sequenced and bioinformatics analyzed by RNA-Seq sequencing technology,and the GO and KEGG functional enrichment analysis were carried out.The results show that in the sgf73Δgene-deficient strains,there are 1834 highly expressed genes,including 6 extremely highly expressed genes,and 1714 differentially expressed genes,of which 934 genes are up-regulated and 780 genes are down-regulated.The sgf73 gene knockout leads to abnormal changes in cellular metabolism and transmembrane transport.The down-regulation of cki1,cki2 and cdc25 genes involved in cycle regulation in the MAPK signaling pathway leads to prolongation of mitotic time in sgf73Δstrain,the down-regulation of regulatory cytoskeleton rgf2,rho1 and stt4 genes leads to abnormal contraction of actin ring of sgf73Δstrain.
作者
刘欣岚
叶姿妤
鲁艳
侯怡铃
周丽倩
蒲帝宏
丁祥
LIU Xinlan;YE Ziyu;LU Yan;HOU Yiling;ZHOU Liqian;PU Dihong;DING Xiang(College of Environmental Science and Engineering,China West Normal University,Nanchong 637009,Sichuan Province,China;Key Laboratory of Southwest China Wildlife Resources Conservation of the Ministry of Education,College of Life Science,China West Normal University,Nanchong 637009,Sichuan Province,China)
出处
《吉林大学学报（理学版）》
CAS
北大核心
2023年第2期426-436,共11页
Journal of Jilin University:Science Edition
基金
四川省科技厅应用基础重点项目(批准号:2018JY0087)
四川省科技厅重点研发项目(批准号:2018NZ0055)
南充市科技局发展计划项目(批准号:20YFZJ0053,20YFZJ0054).
作者简介
第一作者:刘欣岚(1998-),女,汉族,硕士研究生,从事环境微生物学的研究,E-mail:3263407070@qq.com;通信作者:丁祥(1980-),男,汉族,博士,教授,从事环境微生物学的研究,E-mail:biostart8083@126.com.
