摘要
镉(Cd)是一种具有高度细胞毒性的重金属,其生物半衰期长,不易降解,水体中即使很低浓度镉也能对鱼类造成较大损伤。过氧化物酶体增殖物激活受体共激活因子1(peroxisome proliferator activated receptor-γco-activator-1,pgc1)是一种转录共激活因子,其通过激活PPARγ等转录因子活性而参与一系列基因表达调控,在生物能量代谢、线粒体生物合成、抗氧化等生理过程中起着重要的调控作用。为了阐明鳜(Siniperca chuatsi)pgc1基因序列特征、组织表达及重金属镉胁迫对其昼夜节律性表达的影响,本研究对鳜pgc1α、pgc1β基因启动子顺式调控元件、序列特征、组织表达以及水体镉暴露下鳜脑组织中pgc1α和pgc1β基因表达的昼夜节律进行了分析。结果表明,鳜pgc1α和pgc1β启动子中存在NF-E2、IRF1等能量代谢相关转录因子结合位点,pgc1β启动子上存在节律转录因子KLF9结合位点。鳜pgc1α、pgc1β基因都包含完整的LXXLL基序和RRM结构域,与斑马鱼(Danio rerio)pgc1α和pgc1β基因同源性分别为51.6%和59.7%,与人类基因同源性分别为41.5%和28.4%,序列保守性较低。鳜pgc1α和pgc1β基因表达具有明显的组织特异性,均在脑、肾脏、心脏中特异性表达。自然条件下,鳜脑组织中pgc1α和pgc1β表达具有显著的昼夜节律性,均为昼高夜低的表达趋势,其基因表达峰值相位分别为ZT 7.07 h和ZT 8.25 h。重金属镉胁迫导致鳜脑组织pgc1α和pgc1β基因表达昼夜差异减小,振幅下降,基因表达峰值相位分别提前至ZT 3.71 h和ZT 5.65 h,表明水体镉暴露对鳜脑组织pgc1α、pgc1β基因昼夜节律性表达具有显著的扰乱作用。
Cadmium(Cd)is a highly toxic heavy metal element which has a long half-life and is difficult to degrade.Even at very low concentrations,it can cause great damage to fish.Peroxisome proliferator activated receptor-γco-activator-1(pgc1)is a transcriptional co-activator that coordinately regulates the activities of PPARγ,plays an important regulatory role in energy metabolism,mitochondrial biosynthesis,and antioxidative system in organisms.In this study,the cis-regulatory elements,sequence characteristics,evolutionary relationship,tissue expression characteristics of pgc1αand pgc1βgene promoter in Chinese perch,and the circadian rhythm of pgc1αand pgc1βgenes in brain exposed to cadmium were analyzed.The results showed that NF-E2 and IRF1 binding sites existed in the promoter of pgc1αand pgc1β,and KLF9 binding sites existed in the promoter of pgc1β.Both pgc1αand pgc1βgenes contained a complete LXXLL motif and RRM domain.Chinese perch pgc1αand pgc1βshared 51.6%and 59.7%homology with zebrafish and 41.5%and 28.4%homology with human homologous genes,respectively.The expression of pgc1αand pgc1βgenes in Chinese perch has obvious tissue specificity,and are highly expressed in the brain,kidney,and heart.Under natural conditions,the expression of pgc1αand pgc1βin Chinese perch brain showed a trend of being high in the day and low in the night,and the peak phase of gene expression was ZT 7.07 h and ZT 8.25 h,respectively.The diurnal variation of pgc1αand pgc1βgene expression in Chinese perch brain was reduced and the amplitude was decreased under heavy metal cadmium stress.The peak phase of gene expression was advanced to ZT 3.71 h and ZT 5.65 h,respectively,indicating that cadmium exposure causes a significant disturbance on the circadian rhythm of pgc1αand pgc1βin Chinese perch brain.
作者
潘亚雄
陶晋升
张宇
周俊
潘佳琳
唐昭阳
樊轶为
胡名广
李慧菊
黄鑫
褚武英
张建社
PAN Yaxiong;TAO Jinsheng;ZHANG Yu;ZHOU Jun;PAN Jialin;TANG Zhaoyang;FAN Yiwei(HU Mingguang1,LI Huiju1,HUANG Xin1,CHU Wuying1,ZHANG Jianshe11.College of Biological and Environmental Engineering,Changsha University,Changsha 410022,China;College of Marine and Bioengineering,Yancheng Institute of Technology,Yancheng 221051,China)
出处
《中国水产科学》
CAS
CSCD
北大核心
2023年第1期75-85,共11页
Journal of Fishery Sciences of China
基金
国家自然科学基金项目(31820103016,32002364)
湖南省自然科学基金项目(2021JJ40628).
关键词
鳜
pgc1
组织表达
镉暴露
昼夜节律
Siniperca chuatsi
pgc1
tissue expression
cadmium
circadian rhythm
作者简介
潘亚雄(1989-),男,博士,讲师,研究方向为鱼类生理代谢调控.E-mail:biopyx@163.com;通信作者:张建社,教授,研究方向为水生动物营养与品质调控.E-mail:jzhang@ccsu.edu.cn。
