摘要
目的优化地龙酒制工艺。方法采用纤维蛋白平板法检测酶活力;以炮制温度、炮制时间、加酒量和焖润时间为考察因素,以蚯蚓纤溶酶活力为考察指标,采用L_(9)(3^(4))正交试验法优选地龙酒制工艺参数。结果每100kg地龙,加黄酒12.5kg拌匀,焖润30min,70℃翻炒2min时,其酶活力最高(每克31.8万单位)。结论该炮制工艺稳定、可行,可用于地龙的酒制。
Objective To optimize the wine-processing technology of Pheretima.Methods The activity of the enzyme was monitored by the fibrin plate method.With the processing temperature,processing time,alcohol addition and soaking time as the factors,and the activity of earthworm fibrinolytic enzyme as the index,the wine-processing parameters of Pheretima were optimized by the L_(9)(3^(4))orthogonal test.Results For every 100kg of Pheretima,its protease activity was the highest(318000 units per gram)when it was mixed evenly with 12.5kg of yellow rice wine,moistened for 30min,and stir-fried at 70℃ for 2min.Conclusion The processing technology is stable and feasible,which can be used for the wine preparation of Pheretima.
作者
周广涛
黄蒙蒙
解盈盈
汪冰
林永强
ZHOU Guangtao;HUANG Mengmeng;XIE Yingying;WANG Bing;LIN Yongqiang(Shandong Institute for Food and Drug Control·NMPA Key Laboratory of Rubber Product Quality Evaluation·Shandong Engineering Laboratory for Standard Innovation and Quality Evaluation of TCM,Jinan,Shandong,China 250101;Shandong Hongjitang Pharmaceutical Co.,Ltd.,Jinan,Shandong,China 250000)
出处
《中国药业》
CAS
2023年第4期63-65,共3页
China Pharmaceuticals
基金
山东省重点研发计划(重大科技创新工程)[2021CXGC010511]
2021年度山东省人文社会科学课题[2021-YYGL-44]。
关键词
地龙
酒制
炮制工艺
酶活力
正交试验法
纤维蛋白平板法
Pheretima
wine-processing technology
enzyme activity
orthogonal test method
fibrin plate method
作者简介
第一作者:周广涛,男,硕士,主管中药师,研究方向为药品质量标准与评价,(电子信箱)zgt880528@163.com;通信作者:林永强,男,博士,主任药师,研究方向为药品质量标准与评价,(电子信箱)946596315@qq.com。
