摘要
用富硒产朊假丝酵母制备饲料添加剂饲喂家畜家禽可获得富硒农产品。为获取富硒产朊假丝酵母,以产朊假丝酵母为出发菌株,优化摇瓶培养条件(摇瓶装液量、接种量、亚硒酸钠添加量、添加时间和培养液初始p H),通过L9(34)正交试验,获得最佳发酵工艺,即:装液量为35 mL,初始p H为6.5,接种量8%,培养时间为12 h,亚硒酸钠添加量为15μg/mL,此时最终发酵液中总有机硒含量最高,为9 792.732μg/L,菌体有机硒含量为1 400.362μg/g。用获得的高生物量富硒产朊假丝酵母制作饲料添加剂,通过补料培养,获得生物量为19.128 g/L,发酵液中总有机硒量为10 089μg/L。
To obtain selenium-enriched Candida utilis to prepare feed additives to feed livestock and poultry,and obtain selenium-enriched agricultural products,C.utilis was used as the starting strain to optimize the shake flask culture conditions,including the volume of shake flask,the inoculation volume,the amount and time of sodium selenite addition,and the initial pH of the culture fluid.Then the L9(3~4)orthogonal test was done to obtain the optimum fermentation process when liquid volume was 35 mL,the initial pH was 6.5,and the inoculation volume was 8%.When sodium selenite was added at 12 h at 15μg/mL,the total organic selenium content in the final fermentation broth was the highest at 9 792.732μg/L.The content was 1 400.362μg/mL.The fermentor was then cultivated to obtain high biomass selenium-rich C.utilis to make feed additives.Through feed culture,the biomass was 19.128 g/L,and the total organic selenium in the fermentation broth was 10 089μg/L.
作者
马楚楚
陈帅君
赵宝顶
唐伦霞
童应凯
边嘉宾
Ma Chuchu;Chen Shuaijun;Zhao Baoding;Tang Lunxia;Tong Yingkai;Bian Jiabin(College of Agronomy and Resources Environment,Tianjin Agricultural University,Tianjin 300392,China)
出处
《天津农学院学报》
CAS
2022年第4期26-30,35,共6页
Journal of Tianjin Agricultural University
基金
天津市大学生创新创业训练计划项目(201810061122)
天津市一二三产业融合发展科技示范工程项目(18ZXYENC00140)
天津市农村科技帮扶工程项目(18ZXBFNC00330)。
关键词
产朊假丝酵母
富硒
发酵罐
条件优化
Candida utilis
selenium enrichment
fermentation tank
optimization of condition
作者简介
马楚楚(1995—),男,本科在读,研究方向:微生物发酵。E-mail:18222328979@qq.com;通信作者:陈帅君(1982—),女,讲师,硕士,研究方向:生物技术育种。E-mail:86895955@qq.com。
