摘要
目的:运用均匀设计法探究丹皮酚-栀子苷组合对急性酒精性肝损伤的最优配比及配伍效应。方法:采用Lieber-DeCarli酒精液体饲料诱导小鼠急性酒精性肝损伤模型。运用均匀设计法筛选丹皮酚和栀子苷最佳配比剂量组合,并用试剂盒检测肝损伤和氧化应激相关指标,ELISA法检测血清炎症因子水平,Western-blot检测肝脏p38MAPK、JNK、NF-κB P65相关蛋白表达。结果:回归方程提示丹皮酚∶栀子苷=220∶20是二者抗酒精性肝损伤的最优配比。与模型组比较,丹皮酚+栀子苷组肝损伤指标和氧化产物水平明显降低(P<0.05),肝组织抗氧化酶活力明显升高(P<0.05),p-p38MAPK、p-JNK、NF-κB P65蛋白表达水平明显降低(P<0.05)。结论:应用均匀设计和药效分析法可有效优化出丹皮酚-栀子苷最佳配比剂量,两药配伍可通过降低小鼠肝组织氧化应激损伤和炎症反应减轻酒精性肝损伤,其作用可能与靶向p38MAPK/JNK/NF-κB通道有关。
Objective:To explore the optimal ratio and compatibility effect of paeonol-geniposide combination on acute alcoholic liver injury by uniform design. Methods:Lieber-DeCarli alcoholic liquid feed was used to induce acute alcoholic liver injury in mice. Uniform design was used to select the best dosage combination of paeonol and geniposide,and the related indexes of liver injury and oxidative stress were detected by kit. Serum inflammatory factors were detected by ELISA,and the expressions of p38MAPK,JNK and NF-κB P65 related proteins in liver were detected by Western-blot. Results:The regression equation suggested that paeonol:geniposide = 220∶20 was the best ratio of paeonol and geniposide to resist alcoholic liver injury. Compared with the model group,the liver injury indexes and oxidation products of the paeonol+geniposide group decreased significantly,the antioxidant activity of liver tissue increased significantly,and the expression levels of p-p38 MAPK,p-JNK and NF-κB P65 protein decreased significantly. Conclusion:The optimal dosage of paeonol-geniposide was effectively optimized by uniform design and pharmacodynamic analysis. The combination of the two drugs could reduce the alcoholic liver injury by reducing the oxidative stress injury and inflammatory response in the liver tissue of mice,and its effect might be related to the targeting of p38 MAPK/JNK/NF-κB channel.
作者
刘科
刘玉龙
孙敏
刘玲玲
张鲁宁
颜贵明
LIU Ke;LIU Yu-long;SUN Min;LIU Ling-ling;ZHANG Lu-ning;YAN Gui-ming(Anhui University of Chinese Medicine,Hefei 230012,China)
出处
《海南医学院学报》
CAS
2022年第24期1867-1873,共7页
Journal of Hainan Medical University
基金
安徽省自然科学基金资助项目(2008085MH300)。
关键词
丹皮酚
栀子苷
酒精性肝损伤
均匀设计
中药有效成分配比
Paeonol
Geniposide
Alcoholic liver injury
Uniform design
Chinese medicine effective ingredient ratio
作者简介
刘科,E-mail:173428860@qq.com;通讯作者:颜贵明,教授,E-mail:922-119@163.com。
