摘要
目的 建立高效液相色谱一测多评法同步检测藤黄健骨丸中松果菊苷、麦角甾苷、柚皮苷、淫羊藿定A、淫羊藿定B、淫羊藿定C、淫羊藿苷含量的方法。方法 采用高效液相色谱法,Agilent ZORBAX Extend C(4.6mm×250mm,5μm)色谱柱,流动相:乙腈-0.1%磷酸溶液,梯度洗脱;流速1.0 ml/min;检测波长280 nm;柱温为25℃;进样量为10μl。以淫羊藿苷为内参物,建立其他6种成分的相对校正因子,并计算各成分含量。采用外标法测定藤黄健骨丸中7种成分含量与一测多评法计算结果的准确性进行比较。结果 测定了11批藤黄健骨丸中上述成分的含量,7种成分在各自范围内线性关系良好(r≥0.999 7),平均加标回收率为97.70%~99.25%,RSD为0.53%~1.57%(n=6)。一测多评法所测结果与外标法无明显差异。结论 该方法可用于藤黄健骨丸中7种成分含量的同步测定,达到多指标质量控制的目的。
Objective To establish a method of high performance liquid chromatography-quantitative analysis of multi-components by single-marker to simultaneously determine the contents of echinacoside, acteoside, naringin, epimedin A, epimedin B, epimedin C, icariin in Tenghuang Jiangu Pills. Methods The analysis of methanol extract of this drug was performed on a 25℃ thermostatic Agilent ZORBAX Extend C(4.6 mm×250 mm, 5 μm), with the mobile phase comprising of water(containing 0.1% phosphoric acid)-acetonitrile flowing at 1.0 ml/min in a gradient elution manner, and the detection wavelength was set at 280 nm.Using icariin as the internal reference substance, the relative correction factor of the other six components was established and the contents of each component were calculated.In order to validate the accuracy, the seven components in Tenghuang Jiangu Pills were determined and compared by both high performance liquid chromatography-quantitative analysis of multi-components by single-marker and an external standard method. Results The contents of seven components in 11 batches of Tenghuang Jiangu Pills were determined, seven constituents showed good linear relationships within their own ranges(r ≥ 0.999 7), whose average recoveries were 97.70%-99.25% with the RSD of 0.53%-1.57%. The two methods did not show significant difference in assay results. Conclusion The method can be used for the simultaneous determination of seven components in Tenghuang Jiangu Pills, and the purpose of multi-index quality control was achieved.
作者
赵昱玮
南敏伦
司英奇
林宇琪
尹涵
赫玉芳
ZHAO Yuwei;NAN Minlun;SI Yingqi;LING Yuqi;YIN Han;HE Yufang(Preparation Center,Affiliated Hospital of Changchun University of Chinese Medicine,Jilin Province,Changchun130117,China;Institute of Phytochemistry,Jilin Academy of Chinese Medicine Sciences,Jilin Province,Changchun130012,China;Jilin Province AOPU Jinzuan Technology Testing Service Co.,Ltd.,Jilin Province,Changchun130507,China;College of Chinese Medicinal Materials,Jilin Agricultural University,Jilin Province,Changchun130118,China;School of Health Management,Changchun University of Chinese Medicine,Jilin Province,Changchun130117,China)
出处
《中国医药导报》
CAS
2022年第33期33-36,共4页
China Medical Herald
基金
吉林省科技发展计划项目(20200404070YY)。
关键词
高效液相色谱法
一测多评法
藤黄健骨丸
含量测定
High performance liquid chromatography
Quantitative analysis of multi-components by single-marker
Tenghuang Jiangu Pills
Content determination
作者简介
赵昱玮(1983.7-),男,硕士,研究方向:植物化学研究与开发。;通讯作者:赫玉芳(1973.3-),女,博士,教授,研究方向:植物化学研究与开发。
