摘要
目的:建立一种快速、重复性好的疲劳动物模型,以便筛选抗疲劳有效组方。方法:采用食物限制结合游泳复合因素复制小鼠疲劳模型,运用四因素两水平正交设计,以游泳力竭时间、血糖(Glu)含量作为抗疲劳作用指标,运用方差分析揭示A(苁蓉多糖)与B(苁蓉多酚)、C(枸杞多糖)及D(鹿血粉)在抗疲劳中的作用及相互关系,运用直观极差分析发现最佳抗疲劳配方。结果:小鼠在食物限制15 d后,与正常对照组比较,体质量增长缓慢,游泳力竭时间显著缩短,Glu含量显著下降(P<0.05)。采用正交分析发现苁蓉多糖、鹿血粉单独使用均有较好的抗疲劳作用,但相互之间无明显交互作用。采用直接极差判断法发现A1B2C1D1为最佳配方候选。进一步用鹿血多肽E1替代D1进行比较研究发现:组方A1B2C1E1高剂量可显著升高小鼠Glu含量(P<0.05);组方A1B2C1D1高剂量、A1B2C1E1高剂量及低剂量均可显著提高小鼠血清乳酸(LD)含量(P<0.01)。结论:限制食物与游泳结合所复制的小鼠疲劳模型可从多方面阐释受试药物的抗疲劳能力,所得最佳抗疲劳配方为A1B2C1E1(苁蓉多糖0.42 g/kg+苁蓉多酚0.105 g/kg+枸杞多糖0.40 g/kg+鹿血多肽0.17 g/kg),其机理可能是组成成分中含有供能物质,或能够通过某种途径增加机体的产能效能,其相关作用机理有待进一步深入研究。
Objective:To establish a fast and repeatable animal model of fatigue and screen the Chinese medicinal formulations with the anti-fatigue effect.Methods:Food restriction combined with swimming was used to induce a fatigue model in mice.Based on the orthogonal design with four factors and two levels,variance analysis was used to reveal the effect and interaction of Cistanche deserticola polysaccharide(CDPS,A),C.deserticola polyphenol(CDP,B),Lycium barbarum polysaccharide(LBPS,C),and deer blood powder(D)in resisting fatigue with swimming exhausting time and blood glucose(Glu)level as indexes,and range analysis was used to reveal the optimal anti-fatigue Chinese medicinal formulations.Results:Compared with the rats in the normal control group,those receiving 15 days of food restriction showed slow weight gain,shortened swimming exhausting time,and decreased blood Glu.As revealed by the orthogonal analysis,the CDPS and deer blood powder had sound anti-fatigue effects when used alone,but no significant interaction was observed between them.A1 B2 C1 D1 was found to be the optimal formulation candidate by the range analysis.A further comparative study using deer blood peptides(E)instead of deer blood powder showed that the blood Glu level was increased in the high-dose A1 B2 C1 E1 group,and the serum content of lactic acid(LD)was increased in the high-dose A1 B2 C1 D1 group,the high-and low-dose A1 B2 C1 E1 groups(P<0.01).Conclusion:The complex fatigue animal model established by food restriction and swimming can explain the anti-fatigue effect of drugs in multiple ways,and the optimal anti-fatigue formulation is A1 B2 C1 E1(0.42 g/kg A+0.105 g/kg B+0.40 g/kg C+0.17 g/kg E).The mechanism may be attributed to the energy-supplying substances in components or the increase in the body productivity efficiency through some methods,which needs to be further investigated.
作者
张莉
李晓媛
李琳
伍慧
冯希
许婷婷
曾青山
刘晔玮
谭正怀
Zhang Li;Li Xiaoyuan;Li Lin;Wu Hui;Feng Xi;Xu Tingting;Zeng Qingshan;Liu Yewei;Tan Zhenghuai(Sichuan Academy of Chinese Medicine Sciences,Chengdu 610041;Chengdu Jinniu District People's Hospital,Chengdu 610036;Chengdu Shangjin Nanfu Hospital,Chengdu 610730;Chengdu University of TCM,Chengdu 610075;Southwest Medical University,Luzhou 646000;Lanzhou University,Lanzhou 730000)
出处
《中药药理与临床》
CAS
CSCD
北大核心
2022年第3期172-176,共5页
Pharmacology and Clinics of Chinese Materia Medica
基金
甘肃省科技计划项目(编号:20CX9FG238)
2021年四川省中医药重点学科项目——中药药理学(编号:2021ZYYFZZJ-04)
关键词
复合疲劳模型
特色中药
配方筛选
抗疲劳
血糖
血乳酸
Complex fatigue model
characteristic Chinese medicine
Formulation screening
Fatigue resistance
Glucose
Lactic acid
作者简介
通信作者:谭正怀,博士,研究员,从事中药药理与毒理研究,E-mail:tanzhh616@sohu.com;通信作者:刘晔玮,教授,从事药物分析研究,E-mail:liuyw@lzu.edu.cn;张莉,硕士,副研究员,从事中药药理与毒理研究,E-mail:zhangli556@126.com。
