摘要
目的:研究芦荟皮提取物(Aloe bark extract,ABE)对急性酒精性肝损伤的保护效应与机制。方法:首先采用DPPH、ABTS和羟基自由基清除法对ABE的抗氧化活性进行检测;再利用不同浓度(5,10μg/mL)的ABE分别处理酒精诱导HepG2细胞24 h,检测各组细胞天冬氨酸氨基转移酶(AST)、丙氨酸氨基转移酶(ALT)活力和丙二醛(MDA)含量,采用RT-PCR检测其对Nrf2、SOD、ADH、IL-6和IL-1β基因表达的影响;以50%乙醇灌胃雄性C57BL/6J小鼠,造成急性酒精性肝损伤,建立体内动物模型,给予低、中、高剂量组(50、100、200 mg/kg)ABE干预,连续灌胃3 d,检测各组小鼠血清中AST、ALT活力含量,检测肝脏中MDA含量,通过H&E染色观察肝脏病理变化,利用RT-PCR检测肝组织中Nrf2、HO-1、SOD、CAT、ADH和ALDH基因的表达。结果:ABE具有显著的体外抗氧化活性,芦荟皮提取物的DPPH、ABTS和羟基自由基清除能力的IC_(50)值分别为2.03、0.131和0.74 mg/mL。在细胞模型和动物模型中,ABE高、中、低剂量组中细胞和小鼠血清中ALT和AST活性水平显著下降(P<0.05),同时细胞和肝组织中MDA水平也显著降低(P<0.05)。H&E染色结果表明ABE能改善酒精引起的肝脏氧化应激和脂肪变性。分子机制的研究显示其主要通过直接调控酒精代谢相关酶、抗氧化酶和炎症反应起到保肝作用。结论:芦荟皮提取物能够显著缓解酒精引起的肝损伤,是一种潜在的治疗急性酒精性肝损伤的活性原料。
Objective:To explore the protective effect and mechanism of Aloe bark extract(ABE)on acute alcoholic liver injury.Methods:Firstly,the antioxidant activity of ABE was determined by DPPH,ABTS and hydroxyl radical scavenging tests in vitro.Furthermore,different concentrations(5,10μg/mL)of ABE were added to alcohol-induced HepG2 cells for 24 h.The release of cellular aspartate aminotransferase(AST),alanine aminotransferase(ALT)and malondialdehyde(MDA)production were detected,while the Nrf2,SOD,ADH,IL-6 and IL-1βgene expression were detected by RT-PCR.Male C57BL/6J mice were given 50%ethanol by gavage to cause acute alcoholic liver injury,thus an in vivo animal model were established.Various dose groups(50,100,200 mg/kg)were given for three consecutive days,the activity levels of AST and ALT in the serum of the mice in each group were detected,and the content of MDA in the liver was also measured.The pathological changed changes of the liver were observed by H&E staining,and similarly the Nrf2,HO-1,SOD,CAT,ADH and ALDH in liver tissue were measured by RT-PCR.Results:ABE showed significant antioxidant activity in vitro.The IC_(50) values of DPPH,ABTS and hydroxyl radical scavenging activity of ABE were 2.03,0.131 and 0.74 mg/mL,respectively.Within the alcohol-induced cell and animal models,ABE could significantly reduce the levels of ALT and AST in cell and mouse serum(P<0.05),reduce MDA in cells and liver tissue(P<0.05).H&E staining showed that ABE could improve hepatic oxidative stress and steatosis induced by alcohol.Molecular mechanism studies had indicated that ABE play a liver-protecting role mainly by directly regulating alcohol metabolism-related enzymes,antioxidant enzymes and inflammatory responses.Conclusion:The ABE can significantly alleviate alcohol-induced liver injury,and may be potential active substance for treatment of the acute alcoholic liver injury.
作者
褚佳豪
居瑞军
樊远红
吴发亮
彭效明
杨思敏
CHU Jiahao;JU Ruijun;FAN Yuanhong;WU Faliang;PENG Xiaoming;YANG Simin(Beijing Key Laboratory of Enze Biomass Fine Chemicals,Beijing Institute of Petrochemical Technology,Beijing 102617,China)
出处
《食品工业科技》
CAS
北大核心
2023年第1期378-388,共11页
Science and Technology of Food Industry
基金
北京市教委科研计划一般项目(KM201810017002&KM202210017011)
国家自然基金项目(81703453&21406015)
北京石油化工学院校内学科平台建设项目(2019XK006)
北京市优秀人才培养资助项目(2016000020124G058)。
作者简介
褚佳豪(1996-),女,硕士,研究方向:天然产物的提取、纯化及生物活性分析,E-mail:fitachu@163.com。;通信作者:杨思敏(1989-),女,博士,讲师,研究方向:天然产物的药理活性与机制评价,Nrf2与氧化应激及能量代谢等,E-mail:yangsimin@bipt.edu.cn。
