摘要
白粉病是小麦生产中的主要病害之一,发掘抗病基因并实现抗病基因转育是提高作物抗病性的最经济有效的方法。本研究克隆了一个位于小麦染色体1B上、具有典型CC、NBS和LRR结构域的基因TaRPP13-1B。接种白粉菌后,TaRPP13-1B基因在抗病小麦Brock和BJ-1中表达量虽然出现上下调波动,但平均表达水平一直高于感病小麦品种京411。采用病毒诱导的基因沉默和转基因过表达技术进行功能分析,发现抑制目标基因表达,抗病小麦品种Brock对白粉菌的抗性显著降低;过表达TaRPP13-1B的转基因小麦津强5号对白粉菌抗性明显提高。说明TaRPP13-1B基因参与小麦抗白粉病的防御反应过程。该研究为小麦抗病品种的选育提供了有价值的备选基因。
Powdery mildew caused by Blumeria graminis f.sp.tritici(Bgt)is a severe wheat disease in China.Cloning and pyramiding of different resistance genes to improve crop disease resistance is one of the most cost-effective methods.In this study,TaRPP13-1B on chromosome 1B,which encodes the CC,NB-ARC,and LRR domains,was isolated from common wheat.The relative expression level of TaRPP13-1B in Brock and BJ-1 fluctuated after Bgt inoculation,but the average expression levels were always higher than the susceptible wheat Jing 411.The function of TaRPP13-1B was elucidated by virus-induced gene silencing(VIGS)and overexpression transgenic technique.Silencing of TaRPP13-1B resulted in decreased disease resistance in Brock.Overexpression of TaRPP13-1B improved disease resistance in the transgenic wheat seedlings of Jinqiang 5 cultivar.The above results demonstrated that TaRPP13-1B was involved in the defense response of wheat to powdery mildew,which provided valuable genetic resources for breeding of resistant varieties.
作者
刘晓颖
张驰
王雪晴
杨晨晓
王光钰
卞云迪
方芳
王颖
王振英
LIU Xiao-Ying;ZHANG Chi;WANG Xue-Qing;YANG Chen-Xiao;WANG Guang-Yu;BIAN Yun-Di;FANG Fang;WANG Ying;WANG Zhen-Ying(College of Life Sciences,Tianjin Normal University,Tianjin Key Laboratory of Animal and Plant Resistance,Tianjin 300387,China;Forestry Development Service Center of Baodi District,Tianjin 301899,China)
出处
《作物学报》
CAS
CSCD
北大核心
2023年第2期392-401,共10页
Acta Agronomica Sinica
基金
国家自然科学基金项目(31071671)
天津市科技支撑项目(18YFZCNC01100,17JCZDJC34100)资助。
作者简介
通信作者:王振英,E-mail:skywangzy@tjnu.edu.cn;第一作者:刘晓颖,E-mail:skylxy@tjnu.edu.cn。
