摘要
目的探究非小细胞肺癌(non-small cell lung cancer,NSCLC)组织中miR-888-3p的表达及其对患者预后的预测价值.方法对140例NSCLC患者术后切除的癌组织以及配对的癌旁正常肺组织进行研究.以实时荧光定量PCR(real-time quantitative PCR,qRT-PCR)法测定NSCLC组织及癌旁正常肺组织中miR-888-3p的表达情况,并分析其与患者临床病理参数之间的关系;利用TCGA数据库对miR-888-3p在NSCLC的作用进行分析,通过mirtarbase、starbase、mirDIP生物信息学程序数据库对miR-888-3p靶基因数据求交集,并应用Enrichr网站将上述55个靶基因做通路富集分析;采用Kaplan-Meier法分析miR-888-3p水平与NSCLC患者生存情况的关系;采用COX回归分析探讨影响NSCLC患者预后不良的危险因素.结果NSCLC癌组织中miR-888-3p的表达水平显著高于癌旁正常肺组织(P<0.05).miR-888-3p表达水平与肿瘤的分化程度和浸润深度、淋巴结转移、TNM分期明显有关(P<0.05).Kaplan-Meier结果显示,miR-888-3p高表达组生存率低于低表达组生存率(P<0.05).单因素COX回归分析发现,性别、吸烟、淋巴结转移、TNM分期、miR-888-3p水平均是影响NSCLC患者预后的危险因素(P<0.05);多因素COX回归分析表明,TNM分期、miR-888-3p表达水平是影响NSCLC患者预后不良的危险因素(P<0.05).结论NSCLC患者癌组织中miR-888-3p表达升高,与预后不良有关,miR-888-3p可作为NSCLC患者预后评估的一个潜在标志物.
Objective To explore the expression of miR-888-3p in non-small cell lung cancer(NSCLC)tissues and its predictive value for patients’prognosis.Method A total of 140 NSCLC patients were selected,and the cancer tissues removed after operation and paired normal lung tissues adjacent to cancer were taken for research.Real-time quantitative PCR(qRT-PCR)was used to determine the expression of miR-888-3p in NSCLC tissues and adjacent normal lung tissues,and to analyze its relationship with the clinical pathological parameters of the patient.The role of miR-888-3p in NSCLC was studied and analyzed by TCGA database,the intersection of miR-888-3p target gene data was obtained by mirtarbase,starbase and mirDIP bioinformatics program database,and the above 55 target genes were analyzed by Enrichr website.Kaplan-Meier method was used to analyze the relationship between the level of miR-888-3p and the survival of NSCLC patients.COX regression analysis was used to analyze the risk factors affecting the poor prognosis of NSCLC patients.Results The expression level of miR-888-3p in NSCLC cancer tissue was significantly higher than that in adjacent normal lung tissue(P<0.05).The expression level of miR-888-3p was significantly related to the degree of differentiation and depth of invasion,lymph node metastasis and TNM stage(P<0.05).Kaplan-Meier results showed that the cumulative survival rate of patients in the miR-888-3p high-expression group was lower than that in the low-expression group(P<0.05).Univariate COX regression analysis found that gender,smoking,lymph node metastasis,TNM staging,miR-888-3p level were risk factors affecting the prognosis of NSCLC patients(P<0.05);Multivariate COX regression analysis showed that TNM staging and the expression level of miR-888-3p were risk factors affecting the poor prognosis of NSCLC patients(P<0.05).Conclusion The expression of miR-888-3p is elevated in cancer tissues of NSCLC patients,and it is related to poor prognosis.miR-888-3p can be used as a potential marker for prognostic evaluation of NSCLC patients.
作者
窦光华
杨锦雷
张伟
DOU Guanghua;YANG Jinlei;ZHANG Wei(The First Hospital of Hefei,Hefei 230001,China)
出处
《北华大学学报（自然科学版）》
CAS
2022年第5期602-606,共5页
Journal of Beihua University（Natural Science）
基金
安徽省自然科学基金项目(1908085MH203)
合肥市卫生和计划生育委员会基金项目(hwk2016zd001).
作者简介
窦光华(1984—),男,主治医师,主要从事肺癌基础与临床研究,E-mail:douguanghua@126.com;通信作者:张伟(1979—),男,副主任医师,主要从事胸心外科基础和临床研究,E-mail:443712932@qq.com.
