摘要
纸基微流控芯片是一种纸质基底芯片,具有性能优良、价格低廉的优势,然而其制备技术多依赖专业昂贵的设备,限制了纸基微流控芯片的发展。该文采用成本低廉的家用写字机器人,将具有敏感特性的智能水凝胶绘制在纸质基底材料上,得到具有水凝胶阀门的纸基微流控芯片。以Ca^(2+)为模型靶标,该纸基微流控芯片能实现不同浓度(0.1~50 mmol/L)Ca^(2+)溶液的裸眼定量检测,并具有很好的易用性和重现性。同时采用ILX506 CCD传感器制作纸基微流控芯片的数字显示设备,以实现芯片测量结果的自动读取功能。该方法能快速便捷、低成本地制备多条纸基微流控芯片,为其普适化制备提供了新的研究思路,在发展中地区具有良好的推广前景。
The paper-based microfluidic chips are a kind of paper-based chips,which have the ad⁃vantages of excellent performance,low price,safety and portability.However,the preparation technology for paper-based microfluidic chips mostly depends on the professional and expensive equipments,which limits their development to a certain extent.And it is not conducive to the com⁃mercialization process for paper-based microfluidic chips.In this paper,a paper-based microfluidic chip with hydrogel valve was developed by using a low-cost,simple home writing robot to draw a smart hydrogel with sensitive properties on a paper base.In order to verify the feasibility of the prepa⁃ration method,Ca^(2+)was used as a model target.Results showed that the paper-based microfluidic chip prepared by this method could achieve the quantitative detection of Ca^(2+)solutions in the concen⁃tration range of 0.1-50 mmol/L with the naked eye,which has good usability and reproducibility.Meanwhile,a ILX506 CCD sensor was used to design and optimize the digital display device of the paper-based microfluidic chip so as to realize the automatic reading function for the measurement re⁃sults of paper-based microfluidic chip.The experimental results showed that this method could be used for the rapid and convenient preparation of multiple paper-based microfluidic chips at low cost.The preparation method provides a new research idea for the ubiquitous preparation of paper-based mi⁃crofluidic chips,which has a good promotion prospect in those developing regions.
作者
刘旭
高国伟
胡敬芳
张开宇
李延生
LIU Xu;GAO Guo-wei;HU Jing-fang;ZHANG Kai-yu;LI Yan-sheng(Key Laboratory of Sensors,Beijing Information Science and Technology University,Beijing 100101,China;MOE Key Laboratory of Modern Measurement and Control Technology,Beijing Information Science and Technology University,Beijing 100192,China;Joint State Key Laboratory of Sensing Technology,Shanghai Institute of Microsystems and Information Technology,Chinese Academy of Sciences,Shanghai 200050,China)
出处
《分析测试学报》
CAS
CSCD
北大核心
2022年第11期1607-1613,共7页
Journal of Instrumental Analysis
基金
国家自然科学基金项目(62101053)
北京市教育委员会科学研究计划项目(KM202211232004)
传感技术联合国家重点实验室开放课题(SKT1902)。
关键词
纸基微流控芯片
写字机器人
水凝胶
快速检测
可视化定量检测
paper-based microfluidic chip
writing robot
hydrogel
rapid detection
visual quantitative detection
作者简介
通讯作者:李延生,博士研究生,副研究员,研究方向:便携式生化传感器,E-mail:lys2019@bistu.edu.cn。
