摘要
目的建立指纹图谱和3个有效成分含量测定的方法快速筛查苍术混伪品,为苍术药材及饮片的监督检验奠定基础。方法建立58批苍术样品的高效液相色谱(HPLC)指纹图谱,同时测定β-桉叶醇、苍术素、苍术酮的含量,通过计算指纹图谱相似度结合含量测定比值建立筛查苍术混伪品的方法,并对200批苍术国家抽检样品进行检查。结果192批苍术饮片指纹图谱相似度>0.90,苍术酮/苍术素含量比值<2,判断为苍术正品。8批苍术饮片指纹图谱相似度<0.90,苍术酮/苍术素含量比值>200,判断为苍术混伪品。结论该方法灵敏准确,可快速筛查出苍术混伪品,可为苍术药材及饮片质量监管提供有力技术支撑。
Objective To establish a method for rapid screening the adulterants of Atractylodis Rhizoma decoction pieces by fingerprint map and the determination of three active components,and to lay a foundation for the supervision and inspection of Atractylodis Rhizoma crude drug and decoction pieces.Methods The HPLC fingerprints of 58 batches of Atractylodis Rhizoma samples were established,and the contents of β-eudesmol,atractylodin and atractylon were determined simultaneously.The method of screening Atractylodis Rhizoma mixed counterfeits was established by calculating the ratio of fingerprint similarity and content determination.A total of 200 batches of Atractylodis Rhizoma pieces were analyzed by the similarity rate of fingerprints and ratio of content determination.Results A total of 192 batches of samples were identified as authentic Atractylodis Rhizoma pieces by fingerprint similarity(>0.90)and the ratio of Atractylon/Atractylodin(<2).A total of 8 batches of samples were identified as adulterants by fingerprint similarity(<0.90)and the ratio of atractylon/atractylodin(>200).Conclusion This method is sensitive,accurate and can quickly screen out the adulterants of Atractylodis Rhizoma pieces.It will provide a powerful technical support for the quality control of Atractylodis Rhizoma.
作者
彭新航
肖凌
费毅琴
聂晶
PENG Xinhang;XIAO Ling;FEI Yiqin;NIE Jing(College of Pharmacy,Hubei University of Chinese Medicine,Wuhan 430065,China;Hubei Institute for Drug Control,NMPA Key Laboratory for Quality Control of Traditional Chinese Medicine,Wuhan 430075,China)
出处
《医药导报》
CAS
北大核心
2022年第10期1497-1502,共6页
Herald of Medicine
基金
湖北省自然科学基金资助项目(2020CFA024)
湖北省科技重大专项(2020ACA007)。
关键词
苍术饮片
指纹图谱
多成分含量测定
混伪品
快速鉴别
Decoction pieces of Atractylodis Rhizoma
Fingerprint
Determination of multi-component content
Counterfeit
Rapid identification
作者简介
彭新航(1997-),男,湖北黄冈人,在读硕士,研究方向:中药质量评价新技术。ORCID:0000-0001-9851-6192,电话:027-87705272,E-mail:598740485@qq.com;通信作者:费毅琴(1983-),女,湖北武汉人,高级工程师,研究方向:中药质量标准。电话:027-87705271,E-mail:25799556@qq.com;通信作者:聂晶(1970-),女,湖北武汉人,主任药师,博士,研究方向:中药质量评价新技术。ORCID:0000-0002-3429-4110,电话:027-87705232,E-mail:niejingwh@sina.com。
