摘要
【目的】建立文冠果组培苗规模化再生体系,满足文冠果大面积种植需求。【方法】以文冠果茎段为试验材料,采用组织培养的方法对文冠果外植体进行芽诱导,分析不同浓度激素配比对不定芽诱导、增殖、生根的影响,并筛选文冠果组培苗最适移栽基质。【结果】不定芽诱导培养基为MS+0.10 mg/L TDZ(噻苯隆)+1.00 mg/L 6-BA+0.02 mg/L NAA+0.50 mg/L AC(活性炭)时,诱导率最高,为68.57%;增殖培养基为MS+0.5 mg/L 6-BA+0.2 mg/L IBA+0.1 mg/L AC+1.0 mg/L GA3+1.0 g/L Ca(NO_(3))_(2)时,增殖系数最大,为3.20;生根培养基为1/2MS+0.5 mg/L IBA时,生根率最高,为65.56%。珍珠岩、松针土和田园土等比例混合作为移栽基质,文冠果移栽苗成活率显著提高,为88.75%。【结论】试验初步建立了文冠果组织培养再生体系:最佳不定芽培养基为MS+0.10 mg/L TDZ+1.00 mg/L 6-BA+0.02 mg/L NAA+0.50 mg/L AC,最佳增殖培养基为MS+0.5 mg/L 6-BA+0.2 mg/L IBA+0.1 mg/L AC+1.0 mg/L GA3+1.0 g/L Ca(NO_(3))_(2),最佳生根培养基为1/2MS+0.5 mg/L IBA,最佳移栽基质为等比例珍珠岩+松针土+田园土。
【Objective】To address the demands of large-scale planting of Xanthoceras sorbifolia,a large-scale regeneration system using tissue culture seedlings of the plant was constructed.【Methods】The explants of Xanthoceras sorbifolia were induced by tissue culture using the stem segments of the plant as experimental materials.The effects of various hormone doses on the induction,proliferation,and rooting of adventitious buds were examined to determine the optimal medium for Xanthoceras sorbifolia tissue culture.【Results】For adventitious bud induction,MS+0.10 mg/L TDZ+1.00 mg/L 6-BA+0.02 mg/L NAA+0.50 mg/L AC(activated carbon)medium had the highest induction rate of 68.57%.MS+0.5 mg/L 6-BA+0.2 mg/L IBA+0.1 mg/L AC+1.0 mg/L GA3+1.0 g/L Ca(NO_(3))_(2) medium had the highest proliferation coefficient of 3.20.Rooting medium 1/2MS+0.5 mg/L IBA had the highest rooting rate of 65.56%.Equal proportion mixture of perlite,pine needle soil and pastoral soil as the transplanting medium significantly increased the survival rate of sorbent seedlings,which was 88.75%.【Conclusion】The optimal adventitious bud medium was MS+0.10 mg/L TDZ+1.00 mg/L 6-BA+0.02 mg/L NAA+0.50 mg/L AC.The optimal proliferation medium was MS+0.5 mg/L 6-BA+0.2 mg/L IBA+0.1 mg/L AC+1.0 mg/L GA3+1.0 g/L Ca(NO_(3))_(2).The optimal rooting medium was 1/2MS+0.5 mg/L IBA.And the optimal transplanting medium was equal proportion mixture of perlite,pine needle soil and pastoral soil.
作者
黄卫丽
张文军
张海东
杨跃文
张凤鹤
张雯
HUANG Weili;ZHANG Wenjun;ZHANG Haidong;YANG Yuewen;ZHANG Fenghe;ZHANG Wen(Inner Mongolia Academy of Forestry,Hohhot 010010,China;Inner Mongolia Engineering Laboratory of Economic Forest Sterile Virus-free Cultivation,Hohhot 010010,China)
出处
《北方农业学报》
2022年第4期102-108,共7页
Journal of Northern Agriculture
基金
内蒙古防沙治沙基金会自选项目。
关键词
文冠果
外植体
组织培养
再生体系
Xanthoceras sorbifolia
Explant
Tissue culture
Regeneration system
作者简介
黄卫丽(1984-),女,助理研究员,硕士,主要从事林木组织培养与土壤学的研究工作。
