摘要
为建立半红树植物苦槛蓝组织快繁体系,以幼嫩腋芽作为外植体,采用正交设计等试验方法,研究不同消毒时间、不同类型培养基和不同浓度外源激素等因素对苦槛蓝愈伤组织诱导、不定芽诱导和生根的影响。结果显示:(1)最佳消毒时间为0.1%HgCl2消毒3 min;(2)愈伤组织诱导最佳培养基配方为MS培养基+0.6 mg·L-16-BA+0.06 mg·L-1 NAA+30 g·L-1蔗糖+5.5 g·L-1琼脂,pH值为6.2;(3)不定芽继代培养基配方为MS培养基+0.6 mg·L-16-BA+0.03 mg·L-1 NAA+30 g·L-1蔗糖+5.5 g·L-1琼脂,pH值为6.2;(4)生根诱导培养基配方为1/2 MS培养基+0.3 mg·L-1 NAA+20 g·L-1蔗糖+6.5 g·L-1琼脂,pH值为6.2;(5)将草炭土、蛭石和河沙按照3∶1∶1比例混匀制备成移栽基质,育苗成活率可达到86%。
To establish a rapid in vitro propagation system for mangrove associate species Pentacoelium bontioides,the young axillary bud was used as an external implant to study the effects of different disinfection time,different types of culture medium and different concentrations of exogenous hormones on induction of callus,adventitious buds and rooting.The results showed that:(1)the optimum disinfection method was 0.1%HgCl2 treated for 3 min;(2)The optimum medi⁃um for callus induction was MS medium+0.6 mg·L-16-BA+0.06 mg·L-1 NAA+30 g·L-1 sucrose+5.5 g·L-1 agar,pH 6.2;(3)The optimum medium for the induction of adventitious bud was MS medium+0.6 mg·L-16-BA+0.03 mg·L-1 NAA+30 g·L-1 sucrose+5.5 g·L-1 agar,pH 6.2;(4)The optimum medium for rooting formula was 1/2 MS medium+0.3 mg·L-1 NAA+20 g·L-1 sucrose+6.5 g·L-1 agar,pH 6.2;(5)Seedling survival rate could reach 86%when it was transplanted into substrate consisted of peat soil,vermiculite,and river sand with a ratio of 3∶1∶1.
作者
杨党
舒红锁
杨升
刘星
李钧敏
杜照奎
Yang Dang;Shu Hongsuo;Yang Sheng;Liu Xing;Li Junmin;Du Zhaokui(Zhejiang Key Laboratory for Restoration of Damaged Coastal Ecosystems,Taizhou University,Taizhou 318000,China;School of Life Science,Taizhou University,Taizhou 318000,China;Sanmen Natural Resources and Planning Bureau,Sanmen 317100,China;Zhejiang Institute of Subtropical Crops,Wenzhou 325005,China)
出处
《台州学院学报》
2025年第3期21-28,共8页
Journal of Taizhou University
基金
浙江省“尖兵”项目(2023C02003)
浙江省自然资源厅科技项目(浙自然资厅函[2021]306号:2021-43)
台州市科技计划项目(22nya05)
沿海特色植物创新和利用团队项目(NY202401)。
关键词
苦槛蓝
外源激素
半红树
组织培养
生根
Pentacoelium bontioides
exogenous hormones
mangrove associate species
tissue culture
rooting
作者简介
杨党(1983—),男,浙江台州人,助理工程师,主要从事森林培育研究;通信作者:杜照奎(1979—),男,湖北随州人,副教授,博士,主要从事红树林生态学研究。
