摘要
目的观察高铜饮食对大鼠神经行为功能及海马突触相关蛋白表达的影响。方法30只SD雄性大鼠按照随机数字表法分为对照组、高铜饮食组,每组15只。对照组大鼠喂食普通饲料、普通水,高铜饮食组大鼠喂食硫酸铜含量为1 g/kg的高铜饲料和0.185%浓度的硫酸铜去离子水12周。采用电感耦合等离子体-原子发射光谱法和等离子体-质谱法分别检测大鼠血清、海马中铜的含量,并通过刻板行为实验、旷场实验、Morris水迷宫实验检测大鼠的神经行为功能;Western blot技术检测海马微管相关蛋白2(microtubule associated protein 2,MAP2)、生长相关蛋白43(growth associated protein 43,GAP43)的表达水平。运用SPSS 22.0进行统计分析,两组间比较采用两独立样本t检验。结果与对照组比较,高铜饮食组大鼠血清铜[(1.67±0.69)mg/L、(1.98±0.24)mg/L,t=17.53,P<0.05]及海马组织游离铜[(3.52±1.24)mg/g、(4.78±0.57)mg/g,t=10.34,P<0.05]的含量高,刻板行为评分明显高[(0.29±0.08)分、(2.97±0.72)分,t=14.33,P<0.01],旷场实验中穿行空格数[(153.40±24.73)个、(92.46±19.46)个,t=7.50,P<0.01]和直立次数均少[(19.34±1.98)次、(10.57±2.71)次,t=10.12,P<0.01],Morris水迷宫定位航行实验中平均潜伏期长[(3.14±1.67)s、(8.29±2.26)s,t=7.10,P<0.01],空间探索实验中穿越原平台位置次数少[(7.89±2.48)次、(2.98±1.73)次,t=3.23,P<0.01]。高铜饮食组大鼠较对照组大鼠海马区GAP43[(1.03±0.05)、(0.48±0.02),t=39.56,P<0.05]、MAP2[(0.93±0.05)、(0.30±0.08),t=25.86,P<0.05]蛋白表达明显减少。结论高铜饮食可使大鼠多种神经行为功能指标异常,而海马区神经元突触界面MAP2及GAP43的表达减少可能参与了神经行为功能中学习记忆损害的发生过程。
Objective To observe the effects of high copper diet on neurobehavioral functions and synaptic associated protein expression in hippocampus of rats.Methods Thirty male SD rats were randomly divided into control group and high copper diet group with 15 rats in each group according to the random number table method.The rats in control group were fed with ordinary diet and ordinary water,while the rats in high-copper diet group were fed with high-copper diet containing 1 g/kg copper sulfate and 0.185%copper sulfate deionized water for 12 weeks.The content of copper in serum and hippocampus of rats were detected by inductively coupled plasma-atomic emission spectrometry(ICP-AES)and ICP-mass spectrometry(ICP-MS).The neurobehavioral indicators were detected by stereotypic behavior test,open field test and Morris water maze test.The expression levels of microtubule associated protein 2(MAP2)and growth associated protein 43(GAP43)in hippocampus were detected by Western blot.SPSS 22.0 software was used for statistical analysis,and two independent sample t-test was used for comparison between the two groups.Results Compared with the control group,the content of serum copper((1.67±0.69)mg/L,(1.98±0.24)mg/L,t=17.53,P<0.05)and hippocampal free copper((3.52±1.24)mg/g,(4.78±0.57)mg/g,t=10.34,P<0.05)in the high copper diet group increased significantly,and the stereotypic behavior score increased significantly((0.29±0.08),(2.97±0.72),t=14.33,P<0.01),the number of space crossing in the open field experiment((153.40±24.73)points,(92.46±19.46)points,t=7.50,P<0.01)and the times of standing((19.34±1.98)times,(10.57±2.71)times,t=10.12,P<0.01)were significantly decreased.The average latency in Morris water maze navigation test was significantly prolonged((3.14±1.67)s,(8.29±2.26)s,t=7.10,P<0.01),the number of crossing the original platform position in the space exploration test decreased significantly((7.89±2.48)times,(2.98±1.73)times,t=3.23,P<0.01).Compared with control group,protein levels of GAP43((1.03±0.05),(0.48±0.02),t=39.56,P<0.05)and MAP2((0.93±0.05),(0.30±0.08),t=25.86,P<0.05)of high copper diet group were significantly decreased.Conclusion High copper diet causes abnormality in a variety of neurobehavioral function indexes in rats,and a decrease in expression of MAP2 and GAP43 at the synaptic interface of hippocampal neurons may be involved in the process of learning and memory impairment in the neurobehavioral functions.
作者
金珊
杨文明
刘康文
方向
Jin Shan;Yang Wenming;Liu Kangwen;Fang Xiang(Encephalopathy Center,the First Affiliated Hospital of Anhui University of Chinese Medicine,Hefei 230031,China;Graduate School,Anhui University of Chinese Medicine,Hefei 230038,China)
出处
《中华行为医学与脑科学杂志》
CAS
CSCD
北大核心
2022年第8期673-678,共6页
Chinese Journal of Behavioral Medicine and Brain Science
基金
国家自然科学基金(81973825)
安徽省高校自然科学研究项目(KJ2019A0457)
安徽省自然科学基金(2008085MH264)。
关键词
铜
神经行为功能
学习记忆
生长相关蛋白43
微管相关蛋白2
大鼠
Copper
Neurobehavioral functions
Learning and memory
Growth associated protein 43
Microtubule associated protein 2
Rat
作者简介
通信作者:方向,Email:fxdxx@163.com。
